http://oar.icrisat.org/id/eprint/11585 2020-09-04T12:47:38Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/11585/ Molecular and Physiological Alterations in Chickpea under Elevated CO2 Concentrations Palit, P Ghosh, R Tolani, P Tarafdar, A Chitikineni, A Bajaj, P Sharma, M Kudapa, H Varshney, R K Chickpea Genetics and Genomics Climate Change The present study reports profiling of the elevated carbon dioxide (CO2) concentration responsive global transcriptome in chickpea, along with a combinatorial approach for exploring interlinks between physiological and transcriptional changes, important for the climate change scenario. Various physiological parameters were recorded in two chickpea cultivars (JG 11 and KAK 2) grown in open top chambers under ambient [380 parts per million (ppm)] and two stressed/elevated CO2 concentrations (550 and 700 ppm), at different stages of plant growth. The elevated CO2 concentrations altered shoot and root length, nodulation (number of nodules), total chlorophyll content and nitrogen balance index, significantly. RNA-Seq from 12 tissues representing vegetative and reproductive growth stages of both cultivars under ambient and elevated CO2 concentrations identified 18,644 differentially expressed genes including 9,687 transcription factors (TF). The differential regulations in genes, gene networks and quantitative real-time polymerase chain reaction (qRT-PCR) -derived expression dynamics of stress-responsive TFs were observed in both cultivars studied. A total of 138 pathways, mainly involved in sugar/starch metabolism, chlorophyll and secondary metabolites biosynthesis, deciphered the crosstalk operating behind the responses of chickpea to elevated CO2 concentration. Oxford University Press 2020-05 Article PeerReviewed application/pdf en http://oar.icrisat.org/11585/1/pcaa077.pdf Palit, P and Ghosh, R and Tolani, P and Tarafdar, A and Chitikineni, A and Bajaj, P and Sharma, M and Kudapa, H and Varshney, R K (2020) Molecular and Physiological Alterations in Chickpea under Elevated CO2 Concentrations. Plant and Cell Physiology (TSI), 61 (8). pp. 1449-1463. ISSN 1471-9053 https://doi.org/10.1093/pcp/pcaa077 doi:10.1093/pcp/pcaa077 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage