http://oar.icrisat.org/id/eprint/11582 2020-09-04T12:40:19Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/11582/ Multiomics approach unravels fertility transition in a pigeonpea line for a two‐line hybrid system Pazhamala, L T Chaturvedi, P Bajaj, P Srikanth, S Ghatak, A Chitikineni, A Bellaire, A Hingane, A Kumar, C V S Saxena, K B Weckwerth, W Saxena, R K Varshney, R K Plant Breeding Pigeonpea Genetics and Genomics Food and Nutrition Pigeonpea [Cajanus cajan (L.) Millsp.] is a pulse crop cultivated in the semi-arid regions of Asia and Africa. It is a rich source of protein and capable of alleviating malnutrition, improving soil health and the livelihoods of small-holder farmers. Hybrid breeding has provided remarkable improvements for pigeonpea productivity, but owing to a tedious and costly seed production system, an alternative two-line hybrid technology is being explored. In this regard, an environmentsensitive male sterile line has been characterized as a thermosensitive male sterile line in pigeonpea precisely responding to day temperature. The male sterile and fertile anthers from five developmental stages were studied by integrating transcriptomics, proteomics and metabolomics supported by precise phenotyping and scanning electron microscopic study. Spatio-temporal analysis of anther transcriptome and proteome revealed 17 repressed DEGs/DEPs in sterile anthers that play a critical role in normal cell wall morphogenesis and tapetal cell development. The male fertility to sterility transitionwasmainly due to a perturbation in auxin homeostasis, leading to impaired cellwallmodification and sugar transport. Limited nutrient utilization thus leads to microspore starvation in response to moderately elevated day temperature which could be restored with auxin-treatment in the male sterile line. Our findings outline a molecular mechanism that underpins fertility transition responses thereby providing a process-oriented two-line hybrid breeding framework for pigeonpea. Crop Science Society of America 2020-06 Article PeerReviewed application/pdf en http://oar.icrisat.org/11582/1/tpg2.pdf Pazhamala, L T and Chaturvedi, P and Bajaj, P and Srikanth, S and Ghatak, A and Chitikineni, A and Bellaire, A and Hingane, A and Kumar, C V S and Saxena, K B and Weckwerth, W and Saxena, R K and Varshney, R K (2020) Multiomics approach unravels fertility transition in a pigeonpea line for a two‐line hybrid system. The Plant Genome (TSI), 13 (2). pp. 1-20. ISSN 1940-3372 https://doi.org/10.1002/tpg2.20028 doi:10.1002/tpg2.20028 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage