"11582","11","archive","3170",,,"disk0/00/01/15/82","2020-09-04 12:40:19","2020-09-04 12:40:19","2020-09-04 12:40:19","article",,,"show",,,,"","","","","","","","","","",,,,"Pazhamala","L T","","","","","","Female","Pazhamala","L T","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Multiomics approach unravels fertility transition in a pigeonpea line for a two‐line hybrid system","pub","PLB1","CRPS3","CG1","public",,,"Pigeonpea, Nutrition, Small-holder Farmer, Breeding",,"This study was supported by Blue Sky Research and Systems
Biology Research Initiative from ICRISAT, partially
funded by the US Agency for International Development
(USAID), India Mission and Department of Agriculture
Cooperation & FarmersWelfare, Ministry of Agriculture &
FarmersWelfare, Government of India and Bill & Melinda
Gates Foundation (Tropical Legumes II). Dr. Rajeev K.
Varshney is also thankful to the Science & Engineering
Research Board (SERB) of Department of Science & Technology
(DST), Government of India, for providing the J C
Bose National Fellowship (SB/S9/Z-13/2019). Dr. Arindam
Ghatak was supported by a research grant provided by
Austrian Science Fund (FWF, Der Wissenschaftsfonds; Grant agreement number W1257-20). We would like to
gratefully acknowledge Dr. Vinay Kumar, Ms. Akanksha
Kulshreshtha, Mr. M. Manikyam and Mr. Jaipal Goud
for extending technical support. Thanks are due to Mr.
Joseph Mathews and Mr. Anis Akhtar for assisting in
growth chamber experiments. We extend our thanks
to Dr. L. Krishnamurthy for providing valuable suggestions
on setting up experiments under controlled plant
growth conditions. This work has been undertaken as
part of the CGIAR Research Program on Grain Legumes
and Dryland Cereals (GLDC). ICRISAT is a member of
CGIAR Consortium.","Pigeonpea [Cajanus cajan (L.) Millsp.] is a pulse crop cultivated in the semi-arid
regions of Asia and Africa. It is a rich source of protein and capable of alleviating
malnutrition, improving soil health and the livelihoods of small-holder farmers.
Hybrid breeding has provided remarkable improvements for pigeonpea productivity,
but owing to a tedious and costly seed production system, an alternative
two-line hybrid technology is being explored. In this regard, an environmentsensitive
male sterile line has been characterized as a thermosensitive male sterile
line in pigeonpea precisely responding to day temperature. The male sterile
and fertile anthers from five developmental stages were studied by integrating
transcriptomics, proteomics and metabolomics supported by precise phenotyping
and scanning electron microscopic study. Spatio-temporal analysis of anther
transcriptome and proteome revealed 17 repressed DEGs/DEPs in sterile anthers
that play a critical role in normal cell wall morphogenesis and tapetal cell development.
The male fertility to sterility transitionwasmainly due to a perturbation
in auxin homeostasis, leading to impaired cellwallmodification and sugar transport.
Limited nutrient utilization thus leads to microspore starvation in response
to moderately elevated day temperature which could be restored with auxin-treatment in the male sterile line. Our findings outline a molecular mechanism
that underpins fertility transition responses thereby providing a process-oriented
two-line hybrid breeding framework for pigeonpea.","2020-06","published",,"The Plant Genome (TSI)","13","2","Crop Science Society of America",,"1-20",,,,,,"doi:10.1002/tpg2.20028",,,,,"TRUE",,"1940-3372",,,,,,"","https://doi.org/10.1002/tpg2.20028","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1002%2Ftpg2.20028&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Chaturvedi","P","","",,,,"Female","Bajaj","P","","",,,,,,,,,,,,,,,"Molecular Systems Biology (MOSYS), Department of Functional and Evolutionary Ecology, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, Vienna","Austria",,,"s1.2",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Bajaj","P","","",,,,,"Srikanth","S","","",,,,,,,,,,,,,,,"Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, Vienna","Australia",,,"s2.13",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Srikanth","S","","",,,,"Female","Chitikineni","A","","",,,,,,,,,,,,,,,"Vienna Metabolomics Center (VIME), University of Vienna",,,,"s2.17",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Ghatak","A","","",,,,,"Saxena","R K","","",,,,,,,,,,,,,,,"Institute of Agriculture, University ofWestern Australia, 35 Stirling Highway, Crawley",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Chitikineni","A","","",,,,"Female","Varshney","R K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Saxena","K B","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Weckwerth","W","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Saxena","R K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11582",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Varshney","R K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,