eprintid: 11570
rev_number: 12
eprint_status: archive
userid: 3170
dir: disk0/00/01/15/70
datestamp: 2020-08-26 07:14:26
lastmod: 2020-08-26 07:15:37
status_changed: 2020-08-26 07:14:26
type: article
metadata_visibility: show
creators_name: Sinha, P
creators_name: Singh, V K
creators_name: Saxena, R K
creators_name: Khan, A W
creators_name: Abbai, R
creators_name: Chitikineni, A
creators_name: Desai, A
creators_name: Molla, J
creators_name: Upadhyaya, H D
creators_name: Kumar, A
creators_name: Varshney, R K
creators_gender: Female
creators_gender: Female
icrisatcreators_name: Sinha, P
icrisatcreators_name: Saxena, R K
icrisatcreators_name: Khan, A W
icrisatcreators_name: Chitikineni, A
icrisatcreators_name: Desai, A
icrisatcreators_name: Upadhyaya, H D
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
affiliation: International Rice Research Institute (IRRI),South-Asia Hub,ICRISAT Campus, Patancheru
affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
affiliation: Ghatal Rabindra Satabarsiki MahaVidyalaya, Paschim Medinipur, West Bengal
affiliation: IRRI South Asia Regional Center, Varanasi
country: India
country: Germany
title: Superior haplotypes for haplotype‐based breeding for drought tolerance in pigeonpea ( Cajanus cajan L.)
ispublished: pub
subjects: DT1
subjects: PLB1
subjects: s1.2
subjects: s2.13
divisions: CRPS3
crps: CG1
full_text_status: public
keywords: Candidate gene-based association analysis, Drought tolerance, Haplotype analysis, Haplotype-based breeding, pigeonpea
note: Authors would like to thank the Department of Biotechnology
(DBT), Ministry of Science and Technology, Government of India, Department of Agriculture Cooperation & Farmers Welfare, Ministry of Agriculture, Government of India and Bill & Melinda
Gates Foundation (in part) for financial support. This work has
been undertaken as part of the CGIAR Research Program on
Grain Legumes and Dryland Cereals. ICRISAT is a member of
CGIAR Consortium.
abstract: Haplotype-based breeding, a recent promising breeding approach to develop tailor-made crop
varieties, deals with identification of superior haplotypes and their deployment in breeding
programmes. In this context, whole genome re-sequencing data of 292 genotypes from
pigeonpea reference set were mined to identify the superior haplotypes for 10 droughtresponsive
candidate genes. A total of 83, 132 and 60 haplotypes were identified in breeding
lines, landraces and wild species, respectively. Candidate gene-based association analysis of
these 10 genes on a subset of 137 accessions of the pigeonpea reference set revealed 23 strong
marker-trait associations (MTAs) in five genes influencing seven drought-responsive component
traits. Haplo-pheno analysis for the strongly associated genes resulted in the identification of
most promising haplotypes for three genes regulating five component drought traits. The
haplotype C. cajan_23080-H2 for plant weight (PW), fresh weight (FW) and turgid weight (TW),
the haplotype C. cajan_30211-H6 for PW, FW, TW and dry weight (DW), the haplotype
C. cajan_26230-H11 for FW and DW and the haplotype C. cajan_26230-H5 for relative water
content (RWC) were identified as superior haplotypes under drought stress condition.
Furthermore, 17 accessions containing superior haplotypes for three drought-responsive genes
were identified. The identified superior haplotypes and the accessions carrying these superior
haplotypes will be very useful for deploying haplotype-based breeding to develop nextgeneration
tailor-made better drought-responsive pigeonpea cultivars.
date: 2020-05
date_type: published
publication: Plant Biotechnology Journal (TSI)
publisher: Wiley Open Access
pagerange: 1-9
id_number: doi:10.1111/pbi.13422
refereed: TRUE
issn: 1467-7644
official_url: https://doi.org/10.1111/pbi.13422
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1111%2Fpbi.13422&btnG=
related_url_type: pub
citation:   Sinha, P and Singh, V K and Saxena, R K and Khan, A W and Abbai, R and Chitikineni, A and Desai, A and Molla, J and Upadhyaya, H D and Kumar, A and Varshney, R K  (2020) Superior haplotypes for haplotype‐based breeding for drought tolerance in pigeonpea ( Cajanus cajan L.).  Plant Biotechnology Journal (TSI).  pp. 1-9.  ISSN 1467-7644     
document_url: http://oar.icrisat.org/11570/1/pbi.13422.pdf