@article{icrisat11570,
           month = {May},
           title = {Superior haplotypes for haplotype?based breeding for drought tolerance in pigeonpea ( Cajanus cajan L.)},
       publisher = {Wiley Open Access},
          author = {P Sinha and V K Singh and R K Saxena and A W Khan and R Abbai and A Chitikineni and A Desai and J Molla and H D Upadhyaya and A Kumar and R K Varshney},
           pages = {1--9},
            year = {2020},
            note = {Authors would like to thank the Department of Biotechnology
(DBT), Ministry of Science and Technology, Government of India, Department of Agriculture Cooperation \& Farmers Welfare, Ministry of Agriculture, Government of India and Bill \& Melinda
Gates Foundation (in part) for financial support. This work has
been undertaken as part of the CGIAR Research Program on
Grain Legumes and Dryland Cereals. ICRISAT is a member of
CGIAR Consortium.},
         journal = {Plant Biotechnology Journal (TSI)},
        keywords = {Candidate gene-based association analysis, Drought tolerance, Haplotype analysis, Haplotype-based breeding, pigeonpea},
             url = {http://oar.icrisat.org/11570/},
        abstract = {Haplotype-based breeding, a recent promising breeding approach to develop tailor-made crop
varieties, deals with identification of superior haplotypes and their deployment in breeding
programmes. In this context, whole genome re-sequencing data of 292 genotypes from
pigeonpea reference set were mined to identify the superior haplotypes for 10 droughtresponsive
candidate genes. A total of 83, 132 and 60 haplotypes were identified in breeding
lines, landraces and wild species, respectively. Candidate gene-based association analysis of
these 10 genes on a subset of 137 accessions of the pigeonpea reference set revealed 23 strong
marker-trait associations (MTAs) in five genes influencing seven drought-responsive component
traits. Haplo-pheno analysis for the strongly associated genes resulted in the identification of
most promising haplotypes for three genes regulating five component drought traits. The
haplotype C. cajan\_23080-H2 for plant weight (PW), fresh weight (FW) and turgid weight (TW),
the haplotype C. cajan\_30211-H6 for PW, FW, TW and dry weight (DW), the haplotype
C. cajan\_26230-H11 for FW and DW and the haplotype C. cajan\_26230-H5 for relative water
content (RWC) were identified as superior haplotypes under drought stress condition.
Furthermore, 17 accessions containing superior haplotypes for three drought-responsive genes
were identified. The identified superior haplotypes and the accessions carrying these superior
haplotypes will be very useful for deploying haplotype-based breeding to develop nextgeneration
tailor-made better drought-responsive pigeonpea cultivars.}
}