eprintid: 11548
rev_number: 10
eprint_status: archive
userid: 3170
dir: disk0/00/01/15/48
datestamp: 2020-08-09 14:22:46
lastmod: 2020-09-03 03:38:39
status_changed: 2020-08-09 14:22:46
type: article
metadata_visibility: show
creators_name: Tamiru, A
creators_name: Paliwal, R
creators_name: Manthi, S J
creators_name: Odeny, D A
creators_name: Midega, C A O
creators_name: Khan, Z R
creators_name: Pickett, J A
creators_name: Bruce, T J A
icrisatcreators_name: Manthi, S J
icrisatcreators_name: Odeny, D A
affiliation: International Centre of Insect Physiology and Ecology (ICIPE),Nairobi
affiliation: ICRISAT (Nairobi)
affiliation: International Institute of Tropical Agriculture (IITA),Ibadan, Nigeria
affiliation: School of Chemistry, Cardiff University, Cardiff
affiliation: School of Life Sciences, Keele University, Staffordshire
country: Kenya
country: Nigeria
country: UK
title: Genome wide association analysis of a stemborer egg induced “call-for-help” defence trait in maize
ispublished: pub
subjects: s2.13
subjects: s2.6
divisions: CRPS5
divisions: CRPS3
full_text_status: public
keywords: Genomics, Maize
note: This Research was supported by BBSRC grant BB/J011371/1. We gratefully acknowledge the financial support
for icipe research by European Union, UK’s Department for International Development (DFID); Swedish International
Development Cooperation Agency (Sida); the Swiss Agency for Development and Cooperation (SDC);
Federal Democratic Republic of Ethiopia and the Kenyan Government. We thank CIMMYT and Prof Mathews
Dida of Maseno University for supplying seed. We thank Amos Gadi and Silas Ouko for assistance with insect
rearing and screen house operations.
abstract: Tritrophic interactions allow plants to recruit natural enemies for protection against herbivory. Here
we investigated genetic variability in induced responses to stemborer egg-laying in maize Zea mays
(L.) (Poaceae). We conducted a genome wide association study (GWAS) of 146 maize genotypes
comprising of landraces, inbred lines and commercial hybrids. Plants were phenotyped in bioassays
measuring parasitic wasp Cotesia sesamiae (Cameron) (Hymenoptera: Braconidae) attraction
to volatiles collected from plants exposed to stemborer Chilo partellus (Swinhoe) (Lepidoptera:
Crambidae) eggs. Genotyping-by-sequencing was used to generate maize germplasm SNP data for
GWAS. The egg-induced parasitoid attraction trait was more common in landraces than in improved
inbred lines and hybrids. GWAS identified 101 marker-trait associations (MTAs), some of which were
adjacent to genes involved in the JA-defence pathway (opr7, aos1, 2, 3), terpene biosynthesis (fps3,
tps2, 3, 4, 5, 7, 9, 10), benzoxazinone synthesis (bx7, 9) and known resistance genes (e.g. maize insect
resistance 1, mir1). Intriguingly, there was also association with a transmembrane protein kinase
that may function as a receptor for the egg elicitor and other genes implicated in early plant defence
signalling. We report maize genomic regions associated with indirect defence and provide a valuable
resource for future studies of tritrophic interactions in maize. The markers identified may facilitate
selection of indirect defence by maize breeders.
date: 2020-07
date_type: published
publication: Scientific Reports (TSI)
volume: 10
number: 1
publisher: Nature Research
id_number: doi:10.1038/s41598-020-68075-2
refereed: TRUE
issn: 2045-2322
official_url: https://doi.org/10.1038/s41598-020-68075-2
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1038%2Fs41598-020-68075-2&btnG=
related_url_type: org
citation:   Tamiru, A and Paliwal, R and Manthi, S J and Odeny, D A and Midega, C A O and Khan, Z R and Pickett, J A and Bruce, T J A  (2020) Genome wide association analysis of a stemborer egg induced “call-for-help” defence trait in maize.  Scientific Reports (TSI), 10 (1).   ISSN 2045-2322     
document_url: http://oar.icrisat.org/11548/1/s41598-020-68075-2.pdf