"11548","10","archive","3170",,,"disk0/00/01/15/48","2020-08-09 14:22:46","2020-09-03 03:38:39","2020-08-09 14:22:46","article",,,"show",,,,"","","","","","","","","","",,,,"Tamiru","A","","","","","","","Manthi","S J","","","","",,,,,"","",,,,,"","","International Centre of Insect Physiology and Ecology (ICIPE),Nairobi","Kenya","Genome wide association analysis of a stemborer egg induced “call-for-help” defence trait in maize","pub","s2.13","CRPS5","","public",,,"Genomics, Maize",,"This Research was supported by BBSRC grant BB/J011371/1. We gratefully acknowledge the financial support
for icipe research by European Union, UK’s Department for International Development (DFID); Swedish International
Development Cooperation Agency (Sida); the Swiss Agency for Development and Cooperation (SDC);
Federal Democratic Republic of Ethiopia and the Kenyan Government. We thank CIMMYT and Prof Mathews
Dida of Maseno University for supplying seed. We thank Amos Gadi and Silas Ouko for assistance with insect
rearing and screen house operations.","Tritrophic interactions allow plants to recruit natural enemies for protection against herbivory. Here
we investigated genetic variability in induced responses to stemborer egg-laying in maize Zea mays
(L.) (Poaceae). We conducted a genome wide association study (GWAS) of 146 maize genotypes
comprising of landraces, inbred lines and commercial hybrids. Plants were phenotyped in bioassays
measuring parasitic wasp Cotesia sesamiae (Cameron) (Hymenoptera: Braconidae) attraction
to volatiles collected from plants exposed to stemborer Chilo partellus (Swinhoe) (Lepidoptera:
Crambidae) eggs. Genotyping-by-sequencing was used to generate maize germplasm SNP data for
GWAS. The egg-induced parasitoid attraction trait was more common in landraces than in improved
inbred lines and hybrids. GWAS identified 101 marker-trait associations (MTAs), some of which were
adjacent to genes involved in the JA-defence pathway (opr7, aos1, 2, 3), terpene biosynthesis (fps3,
tps2, 3, 4, 5, 7, 9, 10), benzoxazinone synthesis (bx7, 9) and known resistance genes (e.g. maize insect
resistance 1, mir1). Intriguingly, there was also association with a transmembrane protein kinase
that may function as a receptor for the egg elicitor and other genes implicated in early plant defence
signalling. We report maize genomic regions associated with indirect defence and provide a valuable
resource for future studies of tritrophic interactions in maize. The markers identified may facilitate
selection of indirect defence by maize breeders.","2020-07","published",,"Scientific Reports (TSI)","10","1","Nature Research",,,,,,,,"doi:10.1038/s41598-020-68075-2",,,,,"TRUE",,"2045-2322",,,,,,"","https://doi.org/10.1038/s41598-020-68075-2","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1038%2Fs41598-020-68075-2&btnG=","org",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"11548",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Paliwal","R","","",,,,,"Odeny","D A","","",,,,,,,,,,,,,,,"ICRISAT (Nairobi)","Nigeria",,,"s2.6","CRPS3",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11548",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Manthi","S J","","",,,,,,,,,,,,,,,,,,,,,,,"International Institute of Tropical Agriculture (IITA),Ibadan, Nigeria","UK",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11548",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Odeny","D A","","",,,,,,,,,,,,,,,,,,,,,,,"School of Chemistry, Cardiff University, Cardiff",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11548",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Midega","C A O","","",,,,,,,,,,,,,,,,,,,,,,,"School of Life Sciences, Keele University, Staffordshire",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11548",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Khan","Z R","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11548",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Pickett","J A","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"11548",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Bruce","T J A","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,