"11545","8","archive","3170",,,"disk0/00/01/15/45","2020-08-09 09:12:40","2020-08-09 09:12:40","2020-08-09 09:12:40","article",,,"show",,,,"","","","","","","","","","",,,,"Saxena","R K","","","","","",,"Saxena","R K","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","High resolution mapping of restoration of fertility (Rf) by combining large population and high density genetic map in pigeonpea [Cajanus cajan (L.) Millsp]","pub","s1.2","CRPS3","CG1","public",,,"Pigeonpea, Hybrids, Restoration of fertility, Genetic map, QTL, Candidate gene",,"This work has been undertaken as part of the CGIAR Research Program on
Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR
Consortium. Authors would also like to thank Mr. Suryanarayana and Mr.
Sudhakar for the technical help.","Background: Restoration of fertility (Rf) is an important trait for pigeonpea hybrid breeding. Few coarse
quantitative trait locus (QTL) studies conducted in the past identified QTLs with large confidence intervals on the
genetic map and could not provide any information on possible genes responsible for Rf in pigeonpea. Therefore, a
larger population comprising of 369 F2s derived from ICPA 2039 × ICPL 87119 was genotyped with high density
Axiom Cajanus SNP Array with 56 K single nucleotide polymorphism (SNPs) for high resolution mapping of Rf.

Results: A genetic map with 4867 markers was developed and a total of four QTLs for Rf were identified. While one
major effect QTL (qRf8.1) was co-localized with the QTL identified in two previous studies and its size was refined
from 1.2 Mb to 0.41 Mb. Further analysis of qRf8.1 QTL with genome sequence provided 20 genes including two
genes namely flowering locus protein T and 2-oxoglutarate/Fe (II)-dependent dioxygenases (2-ODDs) superfamily
protein with known function in the restoration of fertility.

Conclusion: The qRf8.1 QTL and the potential candidate genes present in this QTL will be valuable for genomicsassisted
breeding and identification of causal genes/nucleotides for the restoration of fertility in the hybrid
breeding program of pigeonpea.","2020-07","published",,"BMC Genomics (TSI)","21","1","BMC",,"1-8",,,,,,"doi:10.1186/s12864-020-06859-6",,,,,"TRUE",,"1471-2164",,,,,,"","https://doi.org/10.1186/s12864-020-06859-6","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1186%2Fs12864-020-06859-6&btnG=","pub",,"Department of Agriculture Cooperation & Farmers Welfare, Ministry of Agriculture & Farmers Welfare, Government ofIndia","Tropical Legumes Project TLII",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"11545",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Molla","J","","",,,,,"Yadav","P","","",,,,,,,,,,,,,,,,,,,"s2.13",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Department of Biotechnology, Government of India, Tropical Legumes Project TLII from Bill and Melinda Gates Foundation",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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"11545",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Varshney","R K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,