http://oar.icrisat.org/id/eprint/11510 2020-05-18T11:40:41Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/11510/ Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance Yu, B Jiang, H Pandey, M K Huang, L Huai, D Zhou, X Kang, Y Varshney, R K Sudini, H Ren, X Luo, H Liu, N Chen, W Guo, J Li, W Ding, Y Jiang, Y Lei, Y Liao, B Groundnut Oilseeds Genetics and Genomics Aflatoxins Aflatoxin B1 (AFB1) and aflatoxin B2 (AFB2) are the most common aflatoxins produced by Aspergillus flavus in peanuts, with high carcinogenicity and teratogenicity. Identification of DNA markers associated with resistance to aflatoxin production is likely to o�er breeders e�cient tools to develop resistant cultivars through molecular breeding. In this study, seeds of 99 accessions of a Chinese peanut mini-mini core collection were investigated for their reaction to aflatoxin production by a laboratory kernel inoculation assay. Two resistant accessions (Zh.h0551 and Zh.h2150) were identified, with their aflatoxin content being 8.11%–18.90% of the susceptible control. The 99 peanut accessions were also genotyped by restriction site-associated DNA sequencing (RAD-Seq) for a genome-wide association study (GWAS). A total of 60 SNP (single nucleotide polymorphism) markers associated with aflatoxin production were detected, and they explained 16.87%–31.70% of phenotypic variation (PVE), with SNP02686 and SNP19994 possessing 31.70% and 28.91% PVE, respectively. Aflatoxin contents of accessions with “AG” (existed in Zh.h0551 and Zh.h2150) and “GG” genotypes of either SNP19994 or SNP02686 were significantly lower than that of “AA” genotypes in the mean value of a three-year assay. The resistant accessions and molecular markers identified in this study are likely to be helpful for deployment in aflatoxin resistance breeding in peanuts. MDPI 2020-03 Article PeerReviewed application/pdf en http://oar.icrisat.org/11510/1/toxins-12-00156-v2.pdf Yu, B and Jiang, H and Pandey, M K and Huang, L and Huai, D and Zhou, X and Kang, Y and Varshney, R K and Sudini, H and Ren, X and Luo, H and Liu, N and Chen, W and Guo, J and Li, W and Ding, Y and Jiang, Y and Lei, Y and Liao, B (2020) Identification of Two Novel Peanut Genotypes Resistant to Aflatoxin Production and Their SNP Markers Associated with Resistance. Toxins (TSI), 12 (156). pp. 1-16. ISSN 2072-6651 https://doi.org/10.3390/toxins12030156 doi:10.3390/toxins12030156 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage