eprintid: 11508
rev_number: 15
eprint_status: archive
userid: 3170
dir: disk0/00/01/15/08
datestamp: 2020-05-15 09:54:42
lastmod: 2020-05-15 09:54:42
status_changed: 2020-05-15 09:54:42
type: article
metadata_visibility: show
creators_name: Wan, L
creators_name: Lei, Y
creators_name: Yan, L
creators_name: Liu, Y
creators_name: Pandey, M K
creators_name: Wan, X
creators_name: Varshney, R K
creators_name: Fang, J
creators_name: Liao, B
icrisatcreators_name: Pandey, M K
icrisatcreators_name: Varshney, R K
affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry  of Agriculture, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, China
affiliation: Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, Nanchang, China
affiliation: Key Laboratory of Crop Physiology Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, China
affiliation: College of Agronomy, Jiangxi Agricultural University, Nanchang, China
affiliation: ICRISAT (Patancheru)
affiliation: School of Plant Biology and Institute of Agriculture, The University of Western, Australia, Crawley, WA, Australia
country: China
country: India
country: Australia
title: Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant
ispublished: pub
subjects: s1.3
subjects: s2.12
subjects: s2.13
subjects: s355
divisions: CRPS3
full_text_status: public
keywords: Groundnut, Legumes, Genetics, Genomics
note: We thank Dr. Juncheng Zhang (Huazhong Agricultural University, China) for
critically revising the English in this paper, as well as Dr. Jianbo Jian (BGI) for
assistance with RNA-seq analyses.
abstract: Background: Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut
can enhance the processing efficiency and quality of peanut oil. An integrative analysis of transcriptomes,
metabolomes and histocytology was performed on wsc mutant and its wild type to investigate the regulatory
mechanisms underlying color pigmentation.
Result: Metabolomes revealed flavonoids were redirected in wsc, while multi-omics analyses of wsc mutant seeds
and testae uncovered WSC influenced the flavonoids biosynthesis in testa as well as suberin formation, glycolysis,
the TCA cycle and amino acid metabolism. The mutation also enhanced plant hormones synthesis and signaling.
Further, co-expression analysis showed that FLS genes co-expressed with MBW complex member genes.
Combining tissue expression patterns, genetic analyses, and the annotation of common DEGs for these three stages
revealed that three testa specific expressed candidate genes, Araip.M7RY3, Aradu.R8PMF and Araip.MHR6K were likely
responsible for the white testa phenotype. WSC might be regulated expression competition between FLS and DFR
by controlling hormone synthesis and signaling as well as the MBW complex.
Conclusions: The results of this study therefore provide both candidate genes and novel approaches that can be
applied to improve peanut with desirable seed coat color and flavonoid quality.
date: 2020-04
date_type: published
publication: BMC Plant Biology (TSI)
volume: 20
number: 1
publisher: BMC
pagerange: 2-19
id_number: doi:10.1186/s12870-020-02383-7
refereed: TRUE
issn: 1471-2229
official_url: https://doi.org/10.1186/s12870-020-02383-7
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1186%2Fs12870-020-02383-7&btnG=
related_url_type: pub
funders: National Natural Science Foundation of China
funders: The Innovation Program of the Chinese Academy of Agricultural Sciences
funders: The Central Public-Interest Scientific Institution Basal Research Fund
funders: China Agriculture Research System
funders: The National Key Research and Development Program of China
funders: The Jiangxi Agriculture Research System
citation:   Wan, L and Lei, Y and Yan, L and Liu, Y and Pandey, M K and Wan, X and Varshney, R K and Fang, J and Liao, B  (2020) Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant.  BMC Plant Biology (TSI), 20 (1).  pp. 2-19.  ISSN 1471-2229     
document_url: http://oar.icrisat.org/11508/1/s12870-020-02383-7.pdf