eprintid: 11476 rev_number: 13 eprint_status: archive userid: 3170 dir: disk0/00/01/14/76 datestamp: 2020-04-10 05:25:32 lastmod: 2020-04-10 05:25:32 status_changed: 2020-04-10 05:25:32 type: article metadata_visibility: show creators_name: Ghosh, R creators_name: Tarafdar, A creators_name: Chobe, D R creators_name: Sharath Chandran, U S creators_name: Rani, S creators_name: Sharma, M creators_gender: Female creators_gender: Female icrisatcreators_name: Ghosh, R icrisatcreators_name: Tarafdar, A icrisatcreators_name: Chobe, D R icrisatcreators_name: Sharath Chandran, U S icrisatcreators_name: Rani, S icrisatcreators_name: Sharma, M affiliation: ICRISAT (Patancheru) country: India title: Diagnostic Techniques of Soil Borne Plant Diseases: Recent Advances and Next Generation Evolutionary Trends ispublished: pub subjects: icm subjects: s2.11 subjects: s35 divisions: CRPS2 crps: CG1 full_text_status: public keywords: Soil-borne, Plant pathogen, Disease diagnosis, Next generation, Advance techniques note: The author is thankful to several colleagues at ICRISAT and collaborators from many NARS programs from India. The work reported in this article was undertaken as a part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (CRP–GLDC). ICRISAT is a member of the CGIAR. abstract: All about 80000 diseases have been recorded in plants throughout the world, of them majority are associated with soil-borne diseases. Early, speedy and reliable detection of plant pathogens is prerequisite to optimize suitable and accurate management strategy. Traditionally, the most prevalent techniques used to identify plant pathogens relied upon culture-based morphological approaches; these methods were laborious, time-consuming. Molecular detection strategies could solve these limitations with improved accuracy and reliability. The DNA and protein based pathogen detection techniques such as DNA fingerprinting, biochemical assays, isothermal amplification techniques and serology are gaining importance in rapid soil borne pathogen detection due to their high degree of specificity to distinguish closely related organisms at different taxonomic levels. Here, we review the various molecular tools used for detection of several soil borne plant pathogens and its implementation in agriculture. date: 2019-06 date_type: published publication: Biological Forum – An International Journal volume: 11 number: 2 publisher: Research Trend pagerange: 1-13 refereed: TRUE issn: 2249-3239 official_url: https://hdl.handle.net/20.500.11766/10752 related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Diagnostic+Techniques+of+Soil+Borne+Plant+Diseases%3A+Recent+Advances+and+Next+Generation+Evolutionary+Trends+&btnG= related_url_type: pub funders: The funding support from CGIAR Research Program on Grain Legumes and Dryland Cereals (CRP–GLDC), ICRISAT and SPLICE-Climate Change Program under Department of Science and Technology, Govt. of India [DST/CCP/CoE/142/2018 (G)] is gratefully acknowledged. citation: Ghosh, R and Tarafdar, A and Chobe, D R and Sharath Chandran, U S and Rani, S and Sharma, M (2019) Diagnostic Techniques of Soil Borne Plant Diseases: Recent Advances and Next Generation Evolutionary Trends. Biological Forum – An International Journal, 11 (2). pp. 1-13. ISSN 2249-3239 document_url: http://oar.icrisat.org/11476/1/Ghosh%20et%20al.%202019.pdf