"11404","7","archive","3170",,,"disk0/00/01/14/04","2020-03-18 08:55:37","2020-03-18 08:55:37","2020-03-18 08:55:37","article",,,"show",,,,"","","","","","","","","","",,,,"Sarr","P S","","","","","","","Deshpande","S","","","","",,,,,"","",,,,,"","","Crop, Livestock and Environment Division, Japan International, Research Center for Agricultural Sciences, 1-1 Ohwashi, Tsukuba, shi","Japan","Sorgoleone release from sorghum roots shapes the composition of nitrifying populations, total bacteria, and archaea and determines the level of nitrification","pub","s1.4","CRPS3","","public",,,"Biological nitrification inhibition, Ammonia-oxidizing archaea (AOA), Sorghum, Sorgoleone, Illumina MiSeq",,"This study was implemented under the Japan
International Research Center for Agricultural Sciences (JIRCAS) core
project on Biological Nitrification Inhibition. The authors thank Dr.
Shinya Iwasaki (JIRCAS) for helping to process some of the data in R.","Sorgoleone is a secondary sorghum metabolite released from roots. It has allelopathic properties and is considered to
inhibit ammonia-oxidizing archaea (AOA) and bacteria (AOB) responsible for the rate-limiting step (ammonia oxidation)
in nitrification. Low activity of these microorganisms in soil may contribute to slow down nitrification and reduce
nitrogen loss via denitrification and NO3
− leaching. The potential nitrification rate (PNR) and the composition of
microbial communities were monitored in rhizosphere soil to investigate the growth effect sorghum on biological
nitrification inhibition (BNI). A greenhouse pipe experiment was conducted using sorghum lines IS20205 (highsorgoleone
release ability), IS32234 (medium-sorgoleone release ability), 296B (low-sorgoleone release ability), and a
control (no plants) combined with fertilization application of 0 or 120 kg N ha−1. We applied nitrogen as ammonium
sulfate at 16 days (20 N), 37 days (40 N), and 54 days (60 N). We collected soil solutions at 7.5 cm depths every 3 days
and measured the pH and nitrate levels. At 1 and 2.3 months, we sampled the bulk and rhizosphere soils and roots in the
0–10 cm, 10–30 cm, and 30–80 cm depths to determine NO2, mineral N, total N, total C, sorgoleone, the composition of
AOA, AOB, and total bacteria and archaea. Sorgoleone was continuously released throughout the 2.3 months’ growth
and was significantly higher in IS20205, followed by IS32234 then 296B, which showed shallow levels. The IS2020
5rhizosphere showed lower NO2 and nitrate levels and significant inhibition of AOA populations. However, we did not
find significant differences in the abundance of AOB between plant treatments. Multivariate analysis and Spearman’s
correlations revealed that sorgoleone as well as environmental factors such as soil pH, soil moisture, NO3
−-N, and NH4
+-
N shape the composition of microbial communities. This study demonstrated that the release of higher amounts of
sorgoleone has great potential to inhibit the abundance of AOA and soil nitrification. The breeding of sorghum lines
with the ability to release higher amounts of sorgoleone could be a strategic way to improve the biological nitrification
inhibition during cultivation.","2019-10","published",,"Biology and Fertility of Soils (TSI)","56","2","Springer",,"145-166",,,,,,"doi:10.1007/s00374-019-01405-3",,,,,"TRUE",,"0178-2762",,,,,,"","https://doi.org/10.1007/s00374-019-01405-3","https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1007%2Fs00374-019-01405-3&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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