eprintid: 11398
rev_number: 10
eprint_status: archive
userid: 3170
dir: disk0/00/01/13/98
datestamp: 2020-03-17 05:49:15
lastmod: 2020-03-17 05:51:20
status_changed: 2020-03-17 05:49:15
type: article
metadata_visibility: show
creators_name: Li, S
creators_name: Zhu, Y
creators_name: Varshney, R K
creators_name: Zhan, J
creators_name: Zheng, X
creators_name: Shi, J
creators_name: Wang, X
creators_name: Liu, G
creators_name: Wang, H
icrisatcreators_name: Varshney, R K
affiliation: Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, (Wuhan)
affiliation: Crop Research Institute, Jiangxi Academy of Agricultural Sciences, (Nanchang)
affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, (Wuhan)
affiliation: ICRISAT (Patancheru)
country: China
country: India
title: A systematic dissection of the mechanisms underlying the natural variation of silique number in rapeseed ( Brassica napus L.) germplasm
ispublished: pub
subjects: s2.13
subjects: s3000
divisions: CRPS3
full_text_status: public
keywords: Brassica napusL., Siliquenumber, GWAS, RNA-seq, Leaf area, Photosynthesis
note: This research was supported by the National Key Research andDevelopment Program (2016YFD0100305), the National KeyBasic Research Development Program of China (2015CB150203),the Natural Science Foundation of Hubei Province (2018CFA075),the Natural Science Foundation (31101181), the RapeseedIndustry Technology System (CARS-13), the Agricultural Scienceand Technology Innovation Project (CAAS-ASTIP-2013-OCRI), theCore Research Budget of the Non-profit Governmental ResearchInstitution (1610172017001).
abstract: Silique number is the most important component of yield in rapeseed (Brassica napusL.). Todissect the mechanism underlying the natural variation of silique number in rapeseedgermplasm, a series of studies were performed. A panel of 331 core lines was employed togenome-wide association study (GWAS), and 27 loci (including 20 novel loci) were identified.The silique number difference between the more- and fewer-silique lines can be attributed tothe accumulative differences in flower number and silique setting rate. Each of themaccounted for 75.2% and 24.8%, respectively. The silique number was highly associated withthe total photosynthesis and biomass. Microscopic analysis showed that the differencebetween extremely more- and fewer-silique lines normally occurred at the amount of flowerbud but not morphology. Transcriptome analysis of shoot apical meristem (SAM) suggestedthat most of enriched groups were associated with the auxin biosynthesis/metabolism,vegetative growth and nutrition/energy accumulation. By integrating GWAS and RNA-seqresults, six promising candidate genes were identified, and some of them were related tobiomass accumulation. In conclusion, the natural variation of silique number is largely affectedby the biomass and nutrition accumulation, which essentially reflects the positive regulatoryrelationship between the source and sink. Our study provides a comprehensive and systematicexplanation for natural variation of silique number in rapeseed, which provides a foundationfor its improvement.
date: 2020-01
date_type: published
publication: Plant Biotechnology Journal (TSI)
volume: 18
number: 2
publisher: Wiley Open Access
pagerange: 568-580
id_number: doi:10.1111/pbi.13224
refereed: TRUE
issn: 1467-7644
official_url: https://doi.org/10.1111/pbi.13224
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=A+systematic+dissection+of+the+mechanisms+underlying+the+natural+variation+of+silique+number+in+rapeseed+%28Brassica+napus+L.%29+germplasm&btnG=
related_url_type: pub
citation:   Li, S and Zhu, Y and Varshney, R K and Zhan, J and Zheng, X and Shi, J and Wang, X and Liu, G and Wang, H  (2020) A systematic dissection of the mechanisms underlying the natural variation of silique number in rapeseed ( Brassica napus L.) germplasm.  Plant Biotechnology Journal (TSI), 18 (2).  pp. 568-580.  ISSN 1467-7644     
document_url: http://oar.icrisat.org/11398/1/pbi.13224.pdf