eprintid: 11371
rev_number: 11
eprint_status: archive
userid: 3170
dir: disk0/00/01/13/71
datestamp: 2020-02-14 05:34:01
lastmod: 2020-02-14 05:34:01
status_changed: 2020-02-14 05:34:01
type: article
metadata_visibility: show
creators_name: Gangurde, S S
creators_name: Wang, H
creators_name: Yaduru, S
creators_name: Pandey, M K
creators_name: Fountain, J C
creators_name: Chu, Y
creators_name: Isleib, T
creators_name: Holbrook, C C
creators_name: Xavier, A
creators_name: Culbreath, A K
creators_name: Ozias‐Akins, P
creators_name: Varshney, R K
creators_name: Guo, B
icrisatcreators_name: Gangurde, S S
icrisatcreators_name: Pandey, M K
icrisatcreators_name: Yaduru, S
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
affiliation: Crop Protection and Management Research Unit, USDA-ARS, (Tifton)
affiliation: Department of Plant Pathology, University of Georgia, (Tifton)
affiliation: Horticulture Department, University of Georgia, (Tifton)
affiliation: Department of Crop and Soil Sciences, North Carolina State University, (Raleigh)
affiliation: Crop Genetics and Breeding Research Unit, USDA-ARS, (Tifton)
affiliation: Biostatistics, Corteva Agrisciences, (Johnston)
country: India
country: USA
title: Nested‐association mapping (NAM)‐based genetic dissection uncovers candidate genes for seed and pod weights in peanut ( Arachis hypogaea )
ispublished: pub
subjects: s1.3
subjects: s2.13
subjects: s355
divisions: CRPS3
crps: CG1
full_text_status: public
keywords: Nested-Association Mapping, Pod Weight, Seed Weight, Association Mapping, Linkage Mapping, Candidate Genes, Peanut.
note: We thank Billy Wilson, Xiaohong Guo and Xianyuan Ji for technical assistance in the field and the laboratory. This work is partially supported by the U.S. Department of Agriculture Agricultural Research Service (USDA‐ARS), the Peanut Foundation, the Georgia Peanut Commission, National Peanut Board and CGIAR Research Program on Grain Legumes and Dryland Cereals (CRP‐GLDC) of the CGIAR. SSG acknowledges Department of Science and Technology (DST), Govt. of India, for the award of DST‐INSPIRE fellowship for his Ph.D. research. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.
abstract: Multiparental genetic mapping populations such as nested-association mapping (NAM) havegreat potential for investigating quantitative traits and associated genomic regions leading torapid discovery of candidate genes and markers. To demonstrate the utility and power of thisapproach, two NAM populations, NAM_Tifrunner and NAM_Florida-07, were used for dissectinggenetic control of 100-pod weight (PW) and 100-seed weight (SW) in peanut. Two high-densitySNP-based genetic maps were constructed with 3341 loci and 2668 loci for NAM_Tifrunner andNAM_Florida-07, respectively. The quantitative trait locus (QTL) analysis identified 12 and 8major effect QTLs for PW and SW, respectively, in NAM_Tifrunner, and 13 and 11 major effectQTLs for PW and SW, respectively, in NAM_Florida-07. Most of the QTLs associated with PW andSW were mapped on the chromosomes A05, A06, B05 and B06. A genomewide associationstudy (GWAS) analysis identified 19 and 28 highly significant SNP–trait associations (STAs) inNAM_Tifrunner and 11 and 17 STAs in NAM_Florida-07 for PW and SW, respectively. Thesesignificant STAs were co-localized, suggesting that PW and SW are co-regulated by severalcandidate genes identified on chromosomes A05, A06, B05, and B06. This study demonstratesthe utility of NAM population for genetic dissection of complex traits and performing high-resolution trait mapping in peanut.
date: 2019-12
date_type: published
publication: Plant Biotechnology Journal (TSI)
publisher: Wiley Open Access
pagerange: 1-15
id_number: doi:10.1111/pbi.13311
refereed: TRUE
issn: 1467-7644
official_url: https://doi.org/10.1111/pbi.13311
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=10.1111%2Fpbi.13311&btnG=
related_url_type: pub
citation:   Gangurde, S S and Wang, H and Yaduru, S and Pandey, M K and Fountain, J C and Chu, Y and Isleib, T and Holbrook, C C and Xavier, A and Culbreath, A K and Ozias‐Akins, P and Varshney, R K and Guo, B  (2019) Nested‐association mapping (NAM)‐based genetic dissection uncovers candidate genes for seed and pod weights in peanut ( Arachis hypogaea ).  Plant Biotechnology Journal (TSI).  pp. 1-15.  ISSN 1467-7644     
document_url: http://oar.icrisat.org/11371/1/Gangurde_et_al-2019-Plant_Biotechnology_Journal.pdf