TY  - JOUR
N2  - Multiparental genetic mapping populations such as nested-association mapping (NAM) havegreat potential for investigating quantitative traits and associated genomic regions leading torapid discovery of candidate genes and markers. To demonstrate the utility and power of thisapproach, two NAM populations, NAM_Tifrunner and NAM_Florida-07, were used for dissectinggenetic control of 100-pod weight (PW) and 100-seed weight (SW) in peanut. Two high-densitySNP-based genetic maps were constructed with 3341 loci and 2668 loci for NAM_Tifrunner andNAM_Florida-07, respectively. The quantitative trait locus (QTL) analysis identi?ed 12 and 8major effect QTLs for PW and SW, respectively, in NAM_Tifrunner, and 13 and 11 major effectQTLs for PW and SW, respectively, in NAM_Florida-07. Most of the QTLs associated with PW andSW were mapped on the chromosomes A05, A06, B05 and B06. A genomewide associationstudy (GWAS) analysis identi?ed 19 and 28 highly signi?cant SNP?trait associations (STAs) inNAM_Tifrunner and 11 and 17 STAs in NAM_Florida-07 for PW and SW, respectively. Thesesigni?cant STAs were co-localized, suggesting that PW and SW are co-regulated by severalcandidate genes identi?ed on chromosomes A05, A06, B05, and B06. This study demonstratesthe utility of NAM population for genetic dissection of complex traits and performing high-resolution trait mapping in peanut.
N1  - We thank Billy Wilson, Xiaohong Guo and Xianyuan Ji for technical assistance in the field and the laboratory. This work is partially supported by the U.S. Department of Agriculture Agricultural Research Service (USDA?ARS), the Peanut Foundation, the Georgia Peanut Commission, National Peanut Board and CGIAR Research Program on Grain Legumes and Dryland Cereals (CRP?GLDC) of the CGIAR. SSG acknowledges Department of Science and Technology (DST), Govt. of India, for the award of DST?INSPIRE fellowship for his Ph.D. research. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.
AV  - public
KW  - Nested-Association Mapping
KW  -  Pod Weight
KW  -  Seed Weight
KW  -  Association Mapping
KW  -  Linkage Mapping
KW  -  Candidate Genes
KW  -  Peanut.
A1  - Gangurde, S S
A1  - Wang, H
A1  - Yaduru, S
A1  - Pandey, M K
A1  - Fountain, J C
A1  - Chu, Y
A1  - Isleib, T
A1  - Holbrook, C C
A1  - Xavier, A
A1  - Culbreath, A K
A1  - Ozias?Akins, P
A1  - Varshney, R K
A1  - Guo, B
TI  - Nested?association mapping (NAM)?based genetic dissection uncovers candidate genes for seed and pod weights in peanut ( Arachis hypogaea )
UR  - https://doi.org/10.1111/pbi.13311
JF  - Plant Biotechnology Journal (TSI)
SP  - 1
Y1  - 2019/12//
ID  - icrisat11371
EP  - 15
SN  - 1467-7644
PB  - Wiley Open Access
ER  -