eprintid: 11367
rev_number: 17
eprint_status: archive
userid: 3170
dir: disk0/00/01/13/67
datestamp: 2020-02-12 09:16:50
lastmod: 2020-02-12 09:16:50
status_changed: 2020-02-12 09:16:50
type: article
metadata_visibility: show
creators_name: Sinha, P
creators_name: Singh, V K
creators_name: Saxena, R K
creators_name: Kale, S M
creators_name: Li, Y
creators_name: Garg, V
creators_name: Meifang, T
creators_name: Khan, A W
creators_name: Kim, K D
creators_name: Chitikineni, A
creators_name: Saxena, K B
creators_name: Sameer Kumar, C V
creators_name: Liu, X
creators_name: Xu, X
creators_name: Jackson, S
creators_name: Powell, W
creators_name: Nevo, E
creators_name: Searle, I R
creators_name: Lodha, M
creators_name: Varshney, R K
creators_gender: Female
creators_gender: Female
creators_gender: Female
icrisatcreators_name: Sinha, P
icrisatcreators_name: Saxena, R K
icrisatcreators_name: Garg, V
icrisatcreators_name: Khan, A W
icrisatcreators_name: Chitikineni, A
icrisatcreators_name: Saxena, K B
icrisatcreators_name: Sameer Kumar, C V
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
affiliation: International Rice Research Institute, South-Asia Hub, (Patancheru)
affiliation: The Leibniz Institute of Plant Genetics and Crop Plant Research, (Gatersleben)
affiliation: BGI-Shenzhen, (Shenzhen)
affiliation: University of Georgia, (Athens)
affiliation: Myongji University, (Yongin)
affiliation: Scotland’s Rural College (SRUC), (Edinburgh)
affiliation: University of Haifa, (Haifa)
affiliation: The University of Adelaide, (Adelaide)
affiliation: Centre for Cellular and Molecular Biology (CSIR), (Hyderabad)
country: India
country: Germany
country: China
country: USA
country: Republic of Korea
country: UK
country: Israel
country: Australia
title: Genome‐wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea
ispublished: pub
subjects: s1.2
subjects: s2.13
subjects: s355
divisions: CRPS3
crps: CG1
full_text_status: public
keywords: Epigenomics, Differentially, Expression Gene, Heterosis, Pigeonpea, Small RNA, miRNA
note: This project was financially supported by the Department of Biotechnology (DBT)‐ BioCARe, Government of India (No. BT/PR17931/BIC/101/178/2016), United States Agency for International Development (USAID), Bill and Melinda Gates Foundation and Shenzhen Municipal Government (NO. JCYJ20150529150505656). This work has also been undertaken as part of the CGIAR Research Program on Grain Legumes and Dryland Cereals. ICRISAT is a member of the CGIAR Consortium.
abstract: Hybrids are extensively used in agriculture to deliver an increase in yield, yet the molecular basisof heterosis is not well understood. Global DNA methylation analysis, transcriptome analysis andsmall RNA profiling were aimed to understand the epigenetic effect of the changes in geneexpression level in the two hybrids and their parental lines. Increased DNA methylation wasobserved in both the hybrids as compared to their parents. This increased DNA methylation inhybrids showed that majority of the 24-nt siRNA clusters had higher expression in hybrids thanthe parents. Transcriptome analysis revealed that various phytohormones (auxin and salicylicacid) responsive hybrid-MPV DEGs were significantly altered in both the hybrids in comparison toMPV. DEGs associated with plant immunity and growth were overexpressed whereas DEGsassociated with basal defence level were repressed. This antagonistic patterns of gene expressionmight contribute to the greater growth of the hybrids. It was also noticed that some common aswell as unique changes in the regulatory pathways were associated with heterotic growth inboth the hybrids. Approximately 70% and 67% of down-regulated hybrid-MPV DEGs werefound to be differentially methylated in ICPH 2671 and ICPH 2740 hybrid, respectively. Thisreflected the association of epigenetic regulation in altered gene expressions. Our findings alsorevealed that miRNAs might play important roles in hybrid vigour in both the hybrids byregulating their target genes, especially in controlling plant growth and development, defenceand stress response pathways. The above finding provides an insight into the molecularmechanism of pigeonpea heterosis.
date: 2020-01
date_type: published
publication: Plant Biotechnology Journal (TSI)
publisher: Wiley Open Access
id_number: doi:10.1111/pbi.13333
refereed: TRUE
issn: 1467-7644
official_url: https://doi.org/10.1111/pbi.13333
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Genome-wide+analysis+of+epigenetic+and+transcriptional+changes+associated+with+heterosis+in+pigeonpea&btnG=
related_url_type: pub
citation:   Sinha, P and Singh, V K and Saxena, R K and Kale, S M and Li, Y and Garg, V and Meifang, T and Khan, A W and Kim, K D and Chitikineni, A and Saxena, K B and Sameer Kumar, C V and Liu, X and Xu, X and Jackson, S and Powell, W and Nevo, E and Searle, I R and Lodha, M and Varshney, R K  (2020) Genome‐wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea.  Plant Biotechnology Journal (TSI).   ISSN 1467-7644     
document_url: http://oar.icrisat.org/11367/1/Sinha_et_al-2020-Plant_Biotechnology_Journal.pdf