TY  - JOUR
AV  - public
A1  - Luo, H
A1  - Pandey, M K
A1  - Khan, A W
A1  - Wu, B
A1  - Guo, J
A1  - Ren, X
A1  - Zhou, X
A1  - Chen, Y
A1  - Chen, W
A1  - Huang, L
A1  - Liu, N
A1  - Lei, Y
A1  - Liao, B
A1  - Varshney, R K
A1  - Jiang, H
TI  - Next?generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut ( Arachis hypogaea L.)
UR  - https://doi.org/10.1111/pbi.13153
JF  - Plant Biotechnology Journal (TSI)
SN  - 1467-7644
PB  - Wiley Open Access
N1  - This study was supported by the Youth Chenguang Project of
Science and Technology of Wuhan City (grant number
2017050304010285) and the National Natural Science Foundations of China (grant numbers 31601340, 31571713,
31761143005 and 31461143022). This work has been undertaken
as part of the CGIAR Research Program on Grain Legumes
and Dryland Cereals (GLDC). ICRISAT is a member of CGIAR
Consortium. The funders had no role in study design, data
collection and analysis, decision to publish, or preparation of the manuscript. We thank Prof. Xinyou Zhang from Henan Academy of Agricultural Sciences, China for providing the peanut cultivar Yuanza 5.
N2  - Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350plant species. A few peanut cultivars were found to possess stable and durable bacterial wiltresistance (BWR). Genomics-assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic markers. Here, we deployed sequencing-based trait mappingapproach, QTL-seq, to discover genomic regions, candidate genes and diagnostic markers for BWR in a recombination inbred line population (195 progenies) of peanut. The QTL-seq analysis identified one candidate genomic region on chromosome B02 significantly associated with BWR.Mapping of newly developed single nucleotide polymorphism (SNP) markers narrowed down theregion to 2.07 Mb and confirmed its major effects and stable expressions across three environments. This candidate genomic region had 49 non synonymous SNPs affecting 19 putative candidate genes including seven putative resistance genes (R-genes). Two diagnostic markers were successfully validated in diverse breeding lines and cultivars and could be deployed in genomics-assisted breeding of varieties with enhanced BWR.
KW  - Peanut
KW  -  Bacterial wilt resistance
KW  -  QTL-seq
KW  -  Candidate genes
KW  -  Diagnostic markers
Y1  - 2019/11//
SP  - 2356
ID  - icrisat11336
EP  - 2369
VL  - 17
IS  - 12
ER  -