<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Genome-wide identification of microsatellite markers from cultivated peanut (Arachis hypogaea L.)"^^ . "Background: Microsatellites, or simple sequence repeats (SSRs), represent important DNA variations that are widely\r\ndistributed across the entire plant genome and can be used to develop SSR markers, which can then be used to\r\nconduct genetic analyses and molecular breeding. Cultivated peanut (A. hypogaea L.), an important oil crop\r\nworldwide, is an allotetraploid (AABB, 2n = 4× = 40) plant species. Because of its complex genome, genomic marker\r\ndevelopment has been very challenging. However, sequencing of cultivated peanut genome allowed us to develop\r\ngenomic markers and construct a high-density physical map.\r\nResults: A total of 8,329,496 SSRs were identified, including 3,772,653, 4,414,961, and 141,882 SSRs that were\r\ndistributed in subgenome A, B, and nine scaffolds, respectively. Based on the flanking sequences of the identified\r\nSSRs, a total of 973,984 newly developed SSR markers were developed in subgenome A (462,267), B (489,394), and\r\nnine scaffolds (22,323), with an average density of 392.45 markers per Mb. In silico PCR evaluation showed that an\r\naverage of 88.32% of the SSR markers generated only one in silico-specific product in two tetraploid A. hypogaea\r\nvarieties, Tifrunner and Shitouqi. A total of 39,599 common SSR markers were identified among the two A.\r\nhypogaea varieties and two progenitors, A. duranensis and A. ipaensis. Additionally, an amplification effectiveness of\r\n44.15% was observed by real PCR validation. Moreover, a total of 1276 public SSR loci were integrated with the\r\nnewly developed SSR markers. Finally, a previously known leaf spot quantitative trait locus (QTL), qLLS_T13_A05_7,\r\nwas determined to be in a 1.448-Mb region on chromosome A05. In this region, a total of 819 newly developed\r\nSSR markers were located and 108 candidate genes were detected.\r\nConclusions: The availability of these newly developed and public SSR markers both provide a large number of\r\nmolecular markers that could potentially be used to enhance the process of trait genetic analyses and improve\r\nmolecular breeding strategies for cultivated peanut."^^ . "2019-11" . . . "20" . "1" . . "BMC"^^ . . . "BMC Genomics (TSI)"^^ . . . "14712164" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "X"^^ . "Li"^^ . "X Li"^^ . . "Y"^^ . "Hong"^^ . "Y Hong"^^ . . "Q"^^ . "Lu"^^ . "Q Lu"^^ . . "S"^^ . "Wen"^^ . "S Wen"^^ . . "J"^^ . "Zhang"^^ . "J Zhang"^^ . . "S"^^ . "Li"^^ . "S Li"^^ . . "R K"^^ . "Varshney"^^ . "R K Varshney"^^ . . "G"^^ . "Zhou"^^ . "G Zhou"^^ . . "H"^^ . "Liu"^^ . "H Liu"^^ . . "X"^^ . "Chen"^^ . "X Chen"^^ . . "X"^^ . "Liang"^^ . "X Liang"^^ . . "H"^^ . "Jiang"^^ . "H Jiang"^^ . . "H"^^ . "Lan"^^ . "H Lan"^^ . . "H"^^ . "Li"^^ . "H Li"^^ . . . . . . "Genome-wide identification of microsatellite markers from cultivated peanut (Arachis hypogaea L.) (PDF)"^^ . . . . . "s12864-019-6148-5.pdf"^^ . . . "Genome-wide identification of microsatellite markers from cultivated peanut (Arachis hypogaea L.) (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #11330 \n\nGenome-wide identification of microsatellite markers from cultivated peanut (Arachis hypogaea L.)\n\n" . "text/html" . . . "Groundnut"@en . . . "Oilseeds"@en . . . "Genetics and Genomics"@en . . . "Food and Nutrition"@en . . . "Food Security"@en . . . "Legume Crops"@en . .