eprintid: 11315
rev_number: 21
eprint_status: archive
userid: 3170
dir: disk0/00/01/13/15
datestamp: 2019-09-24 09:19:57
lastmod: 2019-09-24 10:18:21
status_changed: 2019-09-24 09:19:57
type: article
metadata_visibility: show
creators_name: Rezaei, M K
creators_name: Deokar, A A
creators_name: Arganosa, G
creators_name: Roorkiwal, M
creators_name: Pandey, S K
creators_name: Warkentin, T D
creators_name: Varshney, R K
creators_name: Tar′an, B
creators_gender: Female
icrisatcreators_name: Varshney, R K
icrisatcreators_name: Roorkiwal, M
icrisatcreators_name: Pandey, S K
affiliation: Dep. of Plant Sciences, Crop Development Centre, Univ. of Saskatchewan, Saskatoon,(Saskatchewan)
affiliation: ICRISAT (Patancheru)
country: Canada
country: India
title: Mapping Quantitative Trait Loci for Carotenoid Concentration in Three F2 Populations of Chickpea
ispublished: pub
subjects: PG2
subjects: PLB1
subjects: s1.1
subjects: s2.13
divisions: CRPS3
full_text_status: public
keywords: Quantitative trait locus, QTLs, Chickpea seeds, Genomics
note: This study was funded by the Saskatchewan Pulse Growers, the Saskatchewan Ministry of Agriculture, and the Saskatchewan Innovation and Opportunity Scholarship. We thank Parvaneh Hashemi, Carmen Breitkreutz, and Barry Goetz for their technical assistance. We also appreciate support from the Pulse Breeding staff at the University of Saskatchewan.
abstract: Three F2 populations derived from crosses between
cultivars with green and yellow cotyledon colors were used to identify quantitative trait loci (QTLs) associated with carotenoid components in chickpea (Cicer arietinum L.) seeds developed by the Crop Development Centre (CDC). Carotenoids including violaxanthin, lutein, zeaxanthin, b-cryptoxanthin, and b-carotene were assessed in the F2:3 seeds via high-performance liquid chromatography (HPLC). In the ‘CDC Jade’ × ‘CDC Frontier’ population, 1068 bin markers derived from the 50K Axiom CicerSNP array were mapped onto eight linkage groups (LGs). Eight QTLs, including two each for b-carotene and zeaxanthin and
one each for total carotenoids, b-cryptoxanthin, b-carotene, and violaxanthin were identified in this population. In the ‘CDC Cory’ × ‘CDC Jade’ population, 694 bin markers were mapped onto eight LGs and one partial LG. Quantitative trait loci for b-cryptoxanthin, b-carotene, violaxanthin, lutein, and total carotenoids were
identified on LG8. A map with eight LGs was developed from 581 bin markers in the third population derived from the ‘ICC4475’ × ‘CDC Jade’ cross. One QTL for b-carotene and four QTLs, one each for b-cryptoxanthin, b-carotene, lutein, and total carotenoids, were identified in this population. The highest phenotypic variation explained by the QTLs was for b-carotene, which ranged from 58
to 70% in all three populations. A major gene for cotyledon color was mapped on LG8 in each population. A significant positive correlation between cotyledon color and carotenoid concentration was observed. Potential candidate genes associated with carotenoid components were obtained and their locations on the chickpea genome are presented.
date: 2019-11
date_type: published
publication: The Plant Genome (TSI)
volume: 12
number: 3
publisher: Crop Science Society of America
pagerange: 1-12
id_number: 10.3835/plantgenome2019.07.0067
refereed: TRUE
issn: 1940-3372
official_url: https://doi.org/10.3835/plantgenome2019.07.0067
related_url_url: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Mapping+Quantitative+Trait+Loci+for+Carotenoid+Concentration+in+Three+F2+Populations+of+Chickpea&btnG=
related_url_type: pub
funders: Saskatchewan Pulse Growers
funders: The Saskatchewan Ministry of Agriculture
funders: Saskatchewan Innovation and Opportunity Scholarship
citation:   Rezaei, M K and Deokar, A A and Arganosa, G and Roorkiwal, M and Pandey, S K and Warkentin, T D and Varshney, R K and Tar′an, B  (2019) Mapping Quantitative Trait Loci for Carotenoid Concentration in Three F2 Populations of Chickpea.  The Plant Genome (TSI), 12 (3).  pp. 1-12.  ISSN 1940-3372     
document_url: http://oar.icrisat.org/11315/1/tpg-0-0-190067.pdf