"11157","11","archive","1305",,,"disk0/00/01/11/57","2019-07-16 04:21:12","2019-07-16 04:23:37","2019-07-16 04:21:12","article",,,"show",,,,"","","","","","","","","","",,,,"Divya","K","","","","","","Female","Divya","K","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Genome-wide Identification and Characterization of Hsp70 gene family in Pearl millet (Pennisetumglaucum)","pub","ABio","CRPS3","CG1","public",,,"Abiotic stress, Hsps, heat shock proteins, pearl millet, Hsp70 family, Vapour pressure deficit",,"PSR acknowledges the Department of
Science and Technology, Government of India for
the research grant award through INSPIRE Faculty
(Award No. IFA11-LSPA-06). Thanks to SISU
theme for the sequence extraction and
AgriGenome for analyzing the expression data.
This work was undertaken as part of the CGIAR
Research Program on Grain Legumes & Dryland
Cereals (CRP-GLDC). ICRISAT is a member of
the CGIAR Consortium.","Heat shock proteins (Hsps) are a class of
molecular chaperons which are crucial for protein
folding, assembly, and translocation in many
normal cellular processes. They stabilize proteins
and membranes, and can assist in protein
refolding under stress conditions in plants. Pearl
millet (Pennisetum glaucum) is highly abiotic
stress tolerant, but its Hsps have not been
characterized. In the present study, PgHsp70
genes were retrieved and gene information
analyzed in order to characterize their structure,
localization and functions. Genome-wide
screening using the tools of bioinformatics
identified 18 PgHsp70 genes in the pearl millet
genome which have been categorized into four
subfamilies depending on their cellular localization
such as endoplasmic reticulum, mitochondria,
chloroplast and cytoplasm. Number of introns
ranged from 0-11 in PgHsp70 family genes and
the genes are located across 1 to 7 chromosomes.
Phylogenetic analysis of Hsp70s revealed that
they are closely related to Sorghum Hsp70s.
Promoter analysis showed the presence of cisacting elements such as GCN4, HSE, LTR, MBS,
ABRE, MYB, and TC Aassociated with abiotic
stress conditions indicating the involvement of
these genes in the abiotic stress. Under vapour
pressure deficit (VPD) conditions, leaf and root
tissues of VPD-sensitive ICMR 1152 line, showed
mild expression and in the presence of high VPD,
VPD-insensitive ICMR1122 PgHsp70 genes
showed high expression in leaf and root tissues
in comparison with VPD-sensitive line. Gene
PgcHsp70-1 displayed high transcript level under
high VPD conditions. These results expand our
horizon of understanding of the structure and
function of Hsp70s, especially under abiotic stress
conditions which can further be validated and
employed in breeding programs and genetic
engineering.","2019-04","published",,"Current Trends in Biotechnology and Pharmacy","13","2","Association of Biotechnology and Pharmacy",,"102-111",,,,,,,,,,,"TRUE",,"0973-8916",,,,,,"","https://abap.co.in/genome-wide-identification-and-characterization-hsp70-gene-family-pearl-millet-pennisetum-glaucum","https://scholar.google.co.in/scholar?hl=en&as_sdt=0%2C5&q=Genome-wide+Identification+and+Characterization+of+Hsp70+gene+family+in+Pearl+millet+%28Pennisetumglaucum%29&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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"11157",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Bhatnagar-Mathur","P","","",,,,"Female","Reddy","P S","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
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