eprintid: 11130
rev_number: 14
eprint_status: archive
userid: 1305
dir: disk0/00/01/11/30
datestamp: 2019-06-21 06:13:05
lastmod: 2019-06-21 06:13:05
status_changed: 2019-06-21 06:13:05
type: article
metadata_visibility: show
creators_name: Varshney, R K
creators_name: Pandey, M K
creators_name: Bohra, A
creators_name: Singh, V K
creators_name: Thudi, M
creators_name: Saxena, R K
icrisatcreators_name: Varshney, R K
icrisatcreators_name: Pandey, M K
icrisatcreators_name: Thudi, M
icrisatcreators_name: Saxena, R K
affiliation: ICRISAT (Patancheru)
affiliation: ICAR-Indian Institute of Pulses Research (IIPR) (Kanpur)
affiliation: International Rice Research Institute (IRRI), IRRI South Asia Hub, Patancheru (Hyderabad)
country: India
title: Toward the sequence-based breeding in legumes in the post-genome sequencing era
ispublished: pub
subjects: F12
subjects: PLB1
subjects: s1.1
subjects: s1.2
subjects: s1.3
subjects: s2.10
subjects: s2.13
subjects: s26
subjects: s355
divisions: CRPS3
full_text_status: public
keywords: breeding programs, legume crops, chickpea, pigeonpea, groundnut,
genomic tools and technologies, next-generation sequencing technologies,
seed systems, improved varieties, genome sequences, genetic gains,
sequence‑based breeding, speed breeding, genetic diversity,
molecular breeding, trait mapping, genetic populations, genome re‑sequencing
note: The authors are thankful to the Bill and Melinda Gates Foundation (Tropical Legumes I, II and III), United States Agency for International Development (USAID), and MARS-Wrigley of USA; Indian Council of Agricultural Research (ICAR)-National Agricultural Science Funds (NASF), and Department of Biotechnology (DBT) of Government of India and World Bank-assisted Karnataka Watershed Development Project-II (KWDP-II) funded by Government of Karnataka (GoK), India. Authors would like to thank all their colleagues and collaborators at ICRISAT and other organizations for useful discussions about genomics applications in crop improvement. The work reported in this article was undertaken as a part of the CGIAR Research Program on Grain Legumes and Dryland Cereals (GLDC). ICRISAT is a member of the CGIAR.
abstract: Efficiency of breeding programs of legume crops such as chickpea, pigeonpea and groundnut has been considerably improved over the past decade through deployment of modern genomic tools and technologies. For instance, next-generation sequencing technologies have facilitated availability of genome sequence assemblies, re-sequencing of several hundred lines, development of HapMaps, high-density genetic maps, a range of marker genotyping platforms and identification of markers associated with a number of agronomic traits in these legume crops. Although marker-assisted backcrossing and marker-assisted selection approaches have been used to develop superior lines in several cases, it is the need of the hour for continuous population improvement after every breeding cycle to accelerate genetic gain in the breeding programs. In this context, we propose a sequence-based breeding approach which includes use of independent or combination of parental selection, enhancing genetic diversity of breeding programs, forward breeding for early generation selection, and genomic selection using sequencing/genotyping technologies. Also, adoption of speed breeding technology by generating 4–6 generations per year will be contributing to accelerate genetic gain. While we see a huge potential of the sequence-based breeding to revolutionize crop improvement programs in these legumes, we anticipate several challenges especially associated with high-quality and precise phenotyping at affordable costs, data analysis and management related to improving breeding operation efficiency. Finally, integration of improved seed systems and better agronomic packages with the development of improved varieties by using sequence-based breeding will ensure higher genetic gains in farmers’ fields.
date: 2019-03
date_type: published
publication: Theoretical and Applied Genetics (TSI)
volume: 132
number: 3
publisher: Springer
pagerange: 797-816
id_number: 10.1007/s00122-018-3252-x
refereed: TRUE
issn: 0040-5752
official_url: https://doi.org/10.1007/s00122-018-3252-x
related_url_url: https://scholar.google.co.in/scholar?hl=en&as_sdt=0%2C5&q=Toward+the+sequence-based+breeding+in+legumes+in+the+post-genome+sequencing+era&btnG=
related_url_type: pub
citation:   Varshney, R K and Pandey, M K and Bohra, A and Singh, V K and Thudi, M and Saxena, R K  (2019) Toward the sequence-based breeding in legumes in the post-genome sequencing era.  Theoretical and Applied Genetics (TSI), 132 (3).  pp. 797-816.  ISSN 0040-5752     
document_url: http://oar.icrisat.org/11130/1/Toward%20the%20sequence-based%20breeding%20in%20legumes%20in%20the%20post-genome%20sequencing%20era.pdf