eprintid: 11126
rev_number: 9
eprint_status: archive
userid: 1305
dir: disk0/00/01/11/26
datestamp: 2019-06-21 05:26:01
lastmod: 2019-06-21 05:26:01
status_changed: 2019-06-21 05:26:01
type: article
metadata_visibility: show
creators_name: Divisekera, D M W D
creators_name: Samarasekera, J K R R
creators_name: Hettiarachchi, C
creators_name: Gooneratne, J
creators_name: Choudhary, M I
creators_name: Gopalakrishnan, S
creators_gender: Female
creators_gender: Female
icrisatcreators_name: Gopalakrishnan, S
affiliation: Industrial Technology Institute (Colombo)
affiliation: University of Colombo (Colombo)
affiliation: H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi (Karachi)
affiliation: Dr Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi (Karachi)
affiliation: ICRISAT (Patancheru)
country: Sri Lanka
country: Pakistan
country: India
title: Isolation and identification of lactic acid bacteria with probiotic potential from fermented flour of selected banana varieties grown in Sri Lanka
ispublished: pub
subjects: s2
divisions: CRPS2
full_text_status: public
keywords: Fermented banana flour, lactic acid bacteria, probiotics
note: Authors acknowledge the Indo-Sri Lanka Inter-
Governmental Science and Technology Cooperation
Programme for providing the financial assistance
for this study and NAM S&T Centre for awarding joint
NAM - ICCBS post graduate fellowship to DMWD Divisekara
abstract: This study aimed at isolating and identifying lactic acid bacteria (LAB) with probiotic potential from fermented flour of selected banana varieties grown in Sri Lanka and evaluating their probiotic and safety attributes in vitro. Ten lactic acid bacteria were isolated from six varieties of fermented banana flour, kolikuttu, seeni parakum, ambul nadee, ambum, seeni and anamalu. The isolates were screened for phenotypical and biochemical characteristics. The selected isolates were identified by 16 S rRNA sequencing as Enterococcus durans (two strains), E. gallinarum, E. hirae, E. faecium (two strains), Lactobacillus plantarum, L. curieae, Weissella cibaria and Pediococcus acidilactici and their partial sequences were deposited in NCBI. Among them, six isolates were selected based on the results of in vitro safety attributes and evaluated for their probiotic attributes. Three isolates, namely, E. durans MF405179.1, E. faecium MF574466.1 and L. curieae MF405178.1 isolated from kolikuttu, seeni parakum and ambul nadee, respectively demonstrated tolerance to acid, gastric juice, bile, salt, phenol and temperature under gastric conditions, and also showed susceptibility to tested antibiotics. Among the selected isolates, E. durans MF405179.1 demonstrated the highest hydrophobicity and auto-aggregation of 69.91 % and 76.53 %, respectively. Further, it exhibited highest adhesion to both HCT116 and HT29 cell lines demonstrating 72.5 % and 74.16 % adhesion, respectively. This is the first report of isolation and characterisation of LAB strains with probiotic potential from flour of banana varieties kolikuttu, seeni parakum, ambul nadee, ambum, seeni and anamalu grown in Sri Lanka.
date: 2019-03
date_type: published
publication: Journal of the National Science Foundation of Sri Lanka (TSI)
volume: 47
number: 1
publisher: National Science Foundation of Sri Lanka
pagerange: 3-16
id_number: 10.4038/jnsfsr.v47i1.8922
refereed: TRUE
issn: 1391-4588
official_url: https://doi.org/10.4038/jnsfsr.v47i1.8922
related_url_url: https://scholar.google.co.in/scholar?as_ylo=2019&q=isolation+and+identification+of+lactic+acid+bacteria+with+probiotic+potential+from+fermented+flour+of+selected+banana+varieties+grown+in+sri+lanka&hl=en&as_sdt=0,5
related_url_type: pub
citation:   Divisekera, D M W D and Samarasekera, J K R R and Hettiarachchi, C and Gooneratne, J and Choudhary, M I and Gopalakrishnan, S  (2019) Isolation and identification of lactic acid bacteria with probiotic potential from fermented flour of selected banana varieties grown in Sri Lanka.  Journal of the National Science Foundation of Sri Lanka (TSI), 47 (1).  pp. 3-16.  ISSN 1391-4588     
document_url: http://oar.icrisat.org/11126/1/Isolation%20and%20identification%20of%20lactic%20acid%20bacteria%20with%20probiotic%20potential%20from%20fermented%20flour%20of%20selected%20banana%20varieties%20grown%20in%20Sri%20Lanka.pdf