eprintid: 10740
rev_number: 10
eprint_status: archive
userid: 1305
dir: disk0/00/01/07/40
datestamp: 2018-06-13 11:27:57
lastmod: 2019-08-28 06:25:16
status_changed: 2018-06-13 11:27:57
type: conference_item
metadata_visibility: show
creators_name: Somanaboina, A K
creators_name: Narasu, L N
creators_name: Varshney, R K
creators_name: Kavi Kishor, P B
icrisatcreators_name: Varshney, R K
affiliation: Osmania University (Hyderabad)
affiliation: JNTUH (Hyderabad)
affiliation: ICRISAT (Patancheru)
country: India
title: Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous
expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought
stress tolerance
ispublished: pub
subjects: ABio
subjects: s2.13
divisions: CRPS3
full_text_status: public
pres_type: paper
keywords: transgenic tomato, drought stress tolerance
abstract: Tomato (Solanum lycopersicum L.) is the second most produced
and consumed vegetable, next to potato. Pusa early dwarf (PED)
variety of tomato is sensitive to salinity and drought which reduces
the yield and fruit quality in tomato. In the present study,
tomato transgenics were developed with osmotin-like protein
(OLP) and chitinase (Chi11) genes via in vitro and in planta
transformation methods. Transgene integration and transcript
levels were confirmed by multiplex PCR, DNA blot, and multiplex
reverse transcriptase PCR. Homozygous T2 transgenics,
when evaluated for salt and drought stress, showed enhanced
tolerance compared to wild-type plants. Transgenics showed
enhanced root biomass under normal conditions. Transformants
also displayed higher proline content, K+, relative water content,
chlorophyll fluorescence, total biomass, vascular conductivity,
and fruit yield compared to the wild-type plants under stress
conditions. Co-immunoprecipitation revealed that Chi11 co-expresses
with phosphofructokinase2 (PFK2), which may play a
role in enhanced root biomass. qPCR analysis resulted in higher
transcript levels of OLP, Chi11, and PFK2 in transgenics as compared
to the wild-type plants.
date: 2017-02
date_type: published
pagerange: P-233
event_title: InterDrought-V
event_location: Hyderabad, India
event_dates: February 21-25, 2017
event_type: conference
refereed: TRUE
related_url_url: http://idv.ceg.icrisat.org/wp-content/uploads/2017/02/Abstract_Book_Final.pdf
related_url_type: org
citation:   Somanaboina, A K and Narasu, L N and Varshney, R K and Kavi Kishor, P B  (2017) Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought stress tolerance.  In: InterDrought-V, February 21-25, 2017, Hyderabad, India.     
document_url: http://oar.icrisat.org/10740/1/425.pdf