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dc:title "HTML Summary of #10740 \n\nDevelopment of transgenic tomato (Solanum lycoperscicum L.) by heterologous
\nexpression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought
\nstress tolerance\n\n";
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bibo:abstract "Tomato (Solanum lycopersicum L.) is the second most produced\r\nand consumed vegetable, next to potato. Pusa early dwarf (PED)\r\nvariety of tomato is sensitive to salinity and drought which reduces\r\nthe yield and fruit quality in tomato. In the present study,\r\ntomato transgenics were developed with osmotin-like protein\r\n(OLP) and chitinase (Chi11) genes via in vitro and in planta\r\ntransformation methods. Transgene integration and transcript\r\nlevels were confirmed by multiplex PCR, DNA blot, and multiplex\r\nreverse transcriptase PCR. Homozygous T2 transgenics,\r\nwhen evaluated for salt and drought stress, showed enhanced\r\ntolerance compared to wild-type plants. Transgenics showed\r\nenhanced root biomass under normal conditions. Transformants\r\nalso displayed higher proline content, K+, relative water content,\r\nchlorophyll fluorescence, total biomass, vascular conductivity,\r\nand fruit yield compared to the wild-type plants under stress\r\nconditions. Co-immunoprecipitation revealed that Chi11 co-expresses\r\nwith phosphofructokinase2 (PFK2), which may play a\r\nrole in enhanced root biomass. qPCR analysis resulted in higher\r\ntranscript levels of OLP, Chi11, and PFK2 in transgenics as compared\r\nto the wild-type plants."^^xsd:string;
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dct:date "2017-02";
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skos:prefLabel "Abiotic Stress"@en .
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skos:prefLabel "Genetics and Genomics"@en .
dct:title "InterDrought-V"^^xsd:string;
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rdfs:label "Hyderabad, India"^^xsd:string .
foaf:familyName "Somanaboina"^^xsd:string;
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foaf:familyName "Narasu"^^xsd:string;
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foaf:name "L N Narasu"^^xsd:string;
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foaf:familyName "Varshney"^^xsd:string;
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