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        <dc:title>Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous&#13;
expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought&#13;
stress tolerance</dc:title>
        <dc:creator>Somanaboina, A K</dc:creator>
        <dc:creator>Narasu, L N</dc:creator>
        <dc:creator>Varshney, R K</dc:creator>
        <dc:creator>Kavi Kishor, P B</dc:creator>
        <dc:subject>Abiotic Stress</dc:subject>
        <dc:subject>Genetics and Genomics</dc:subject>
        <dc:description>Tomato (Solanum lycopersicum L.) is the second most produced&#13;
and consumed vegetable, next to potato. Pusa early dwarf (PED)&#13;
variety of tomato is sensitive to salinity and drought which reduces&#13;
the yield and fruit quality in tomato. In the present study,&#13;
tomato transgenics were developed with osmotin-like protein&#13;
(OLP) and chitinase (Chi11) genes via in vitro and in planta&#13;
transformation methods. Transgene integration and transcript&#13;
levels were confirmed by multiplex PCR, DNA blot, and multiplex&#13;
reverse transcriptase PCR. Homozygous T2 transgenics,&#13;
when evaluated for salt and drought stress, showed enhanced&#13;
tolerance compared to wild-type plants. Transgenics showed&#13;
enhanced root biomass under normal conditions. Transformants&#13;
also displayed higher proline content, K+, relative water content,&#13;
chlorophyll fluorescence, total biomass, vascular conductivity,&#13;
and fruit yield compared to the wild-type plants under stress&#13;
conditions. Co-immunoprecipitation revealed that Chi11 co-expresses&#13;
with phosphofructokinase2 (PFK2), which may play a&#13;
role in enhanced root biomass. qPCR analysis resulted in higher&#13;
transcript levels of OLP, Chi11, and PFK2 in transgenics as compared&#13;
to the wild-type plants.</dc:description>
        <dc:date>2017-02</dc:date>
        <dc:type>Conference or Workshop Item</dc:type>
        <dc:type>PeerReviewed</dc:type>
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        <dc:language>en</dc:language>
        <dc:identifier>http://oar.icrisat.org/10740/1/425.pdf</dc:identifier>
        <dc:identifier>  Somanaboina, A K and Narasu, L N and Varshney, R K and Kavi Kishor, P B  (2017) Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought stress tolerance.  In: InterDrought-V, February 21-25, 2017, Hyderabad, India.     </dc:identifier></oai_dc:dc>
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