eprintid: 10724
rev_number: 13
eprint_status: archive
userid: 1305
dir: disk0/00/01/07/24
datestamp: 2018-06-13 08:32:59
lastmod: 2018-06-13 08:32:59
status_changed: 2018-06-13 08:32:59
type: conference_item
metadata_visibility: show
creators_name: Mangrauthia, S K
creators_name: Vishnu Prasanth, V
creators_name: Sailaja, B
creators_name: Sarla, N
creators_name: Voleti, S R
creators_name: Subrahmanyam, D
creators_name: Babu, V R
icrisatcreators_name: Sailaja, B
affiliation: Indian Institute of Rice Research (Hyderabad)
affiliation: ICRISAT (Patancheru)
country: India
title: A multi-dimensional approach from seed-to-seed to understand and improve heat stress tolerance in rice
ispublished: pub
subjects: ABio
subjects: CR1
subjects: S2021
full_text_status: public
pres_type: paper
keywords: Heat stress, tolerance, rice
abstract: In changing climatic conditions, stress caused by high temperature
poses a serious threat to rice cultivation. Physiological,
biochemical, and molecular analysis of rice cultivars revealed
that Nagina22 (N22) shows lesser reduction in chlorophyll content,
net photosynthetic rate, spikelet fertility and grain yield,
but increased membrane thermal stability, antioxidant enzymes
activity and transpiration rate (E) at high temperature. DREB,
RAB, LEA, and genes associated with hormones signalling were
induced during germination, while OsFd (an iron sulphur cluster
binding protein) and CWIP (cell wall integrity protein) emerged
as high priority candidate genes in seedling and reproductive
stages. Their function is being analysed by transgene expression
and CRISPR/Cas genome editing approaches. Field screening
in polyhouse, late sowing and temperature gradient chamber
for 20 morpho-physiological traits indicated the importance of
both yield and spikelet fertility, and photosynthesis traits. N22
showed the least Heat Susceptibility Index (HSI) for yield/plant,
spikelet fertility, flag leaf SPAD and stomatal conductance, while
Vandana showed the highest HSI for spikelet fertility and flag leaf
temperature. QTLs for HSI of spikelet fertility were identified on
chromosome 1 and HSI of yield per plant on chromosomes 1, 2, 3,
4, 7 and 8; and PV of 6% to 57% using 174 F2-3 Vandana x N22
mapping population. Simultaneously, RNAseq was performed
to identify the genome wide miRNAs and transcriptome of N22
and Vandana from shoot and root after short and long duration
of heat stress treatments; and recovery phase for an eQTL-guided
function-related co-expression analysis to identify the putative
regulators and gene regulatory networks.
date: 2017-02
date_type: published
pagerange: P-106
event_title: InterDrought-V
event_location: Hyderabad, India
event_dates: February 21-25, 2017
event_type: conference
refereed: TRUE
related_url_url: http://idv.ceg.icrisat.org/wp-content/uploads/2017/02/Abstract_Book_Final.pdf
related_url_type: org
citation:   Mangrauthia, S K and Vishnu Prasanth, V and Sailaja, B and Sarla, N and Voleti, S R and Subrahmanyam, D and Babu, V R  (2017) A multi-dimensional approach from seed-to-seed to understand and improve heat stress tolerance in rice.  In: InterDrought-V, February 21-25, 2017, Hyderabad, India.     
document_url: http://oar.icrisat.org/10724/1/293.pdf