<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Characterization of low Ca2+ stress-induced embryo apoptosis response genes\r\nand their regulation of embryo development"^^ . "Ca2+ deficiency in soil induces early embryo abortion in peanut,\r\nproducing empty pods, or ill-filled pods, especially under\r\ndrought condition, which is a general problem. However, the\r\nunderlying mechanism remains unclear. In this study, embryo\r\nabortion was characterized to be caused by apoptosis marked\r\nwith cell wall degradation. Using a method of SSH cDNA libraries\r\nassociated with library lift (SSHaLL), 62 differentially\r\nexpressed genes were isolated from young peanut embryos,\r\nwhich were classified to be stress responses, catabolic process,\r\ncarbohydrate and lipid metabolism, embryo morphogenesis,\r\nand regulation. The cell retardation with cell wall\r\ndegradation was caused by up-regulated cell wall hydrolases\r\nand down-regulated cellular synthases genes. Two CYP707A4\r\ngenes, encoding abscisic acid (ABA) 8’-hydroxylases, key enzymes\r\nfor ABA catabolism, were up-regulated by 21-fold under\r\nCa2+-deficient conditions, reducing the ABA level in early embryos.\r\nOver-expression of AhCYP707A4 in Nicotiana benthamiana\r\nshowed a phenotype of low ABA content with high numbers\r\nof aborted embryos, small pods and less seeds, which confirms\r\nthat CYP707A4 is a key player in regulation of Ca2+ deficiency-induced\r\nembryo abortion via ABA-mediated apoptosis. The\r\nresults elucidated the mechanism of low Ca2+-induced embryo\r\nabortion. A set of varieties with different sizes of pods were evaluated\r\nfor their tolerance to calcium stress, and we found that\r\nbig pod varieties showed less tolerance to low calcium and more\r\neasy to produce ill-filled pods or empty pods. Drought will make\r\nworse of the phenomenon. Soil supplied with rich calcium will\r\nimprove the pods filling and greatly reduce number of empty\r\npods. We conclude big-pod varieties easier to produce empty\r\npods are also associated with calcium level in the soil."^^ . "2017-02" . . . . . . . . . . . . . . . . . . . . . . . . . . "Y"^^ . "Deng"^^ . "Y Deng"^^ . . "H"^^ . "Chen"^^ . "H Chen"^^ . . "S B"^^ . "Zhou"^^ . "S B Zhou"^^ . . "W J"^^ . "Zhuang"^^ . "W J Zhuang"^^ . . "R K"^^ . "Varshney"^^ . "R K Varshney"^^ . . "T C"^^ . "Cai"^^ . "T C Cai"^^ . . "C"^^ . "Zhang"^^ . "C Zhang"^^ . . . . "InterDrought-V"^^ . . . . . "Hyderabad, India"^^ . . . . . . "Characterization of low Ca2+ stress-induced embryo apoptosis response genes\r\nand their regulation of embryo development (PDF)"^^ . . . . . "Abstract241.pdf"^^ . . . "Characterization of low Ca2+ stress-induced embryo apoptosis response genes\r\nand their regulation of embryo development (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Characterization of low Ca2+ stress-induced embryo apoptosis response genes\r\nand their regulation of embryo development (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Characterization of low Ca2+ stress-induced embryo apoptosis response genes\r\nand their regulation of embryo development (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Characterization of low Ca2+ stress-induced embryo apoptosis response genes\r\nand their regulation of embryo development (Other)"^^ . . . . . . "small.jpg"^^ . . . "Characterization of low Ca2+ stress-induced embryo apoptosis response genes\r\nand their regulation of embryo development (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #10711 \n\nCharacterization of low Ca2+ stress-induced embryo apoptosis response genes \nand their regulation of embryo development\n\n" . "text/html" . . . "Genetics and Genomics"@en . .