"10711","11","archive","1305",,,"disk0/00/01/07/11","2018-06-12 10:50:15","2018-06-12 10:50:15","2018-06-12 10:50:15","conference_item",,,"show",,,,"","","","","","","","","","",,,,"Chen","H","","","","","","","Varshney","R K","","","","",,,,,"","",,,,,"","","State Key Laboratory of Ecological Pest Control for Fujian and Taiwan crops, Fujian Agriculture and Forestry University, Fuzhou (Fujian)","China","Characterization of low Ca2+ stress-induced embryo apoptosis response genes
and their regulation of embryo development","pub","s2.13","CRPS3","","public",,"paper","Ca2+ deficiency, calcium level, soil, embryo development",,,"Ca2+ deficiency in soil induces early embryo abortion in peanut,
producing empty pods, or ill-filled pods, especially under
drought condition, which is a general problem. However, the
underlying mechanism remains unclear. In this study, embryo
abortion was characterized to be caused by apoptosis marked
with cell wall degradation. Using a method of SSH cDNA libraries
associated with library lift (SSHaLL), 62 differentially
expressed genes were isolated from young peanut embryos,
which were classified to be stress responses, catabolic process,
carbohydrate and lipid metabolism, embryo morphogenesis,
and regulation. The cell retardation with cell wall
degradation was caused by up-regulated cell wall hydrolases
and down-regulated cellular synthases genes. Two CYP707A4
genes, encoding abscisic acid (ABA) 8’-hydroxylases, key enzymes
for ABA catabolism, were up-regulated by 21-fold under
Ca2+-deficient conditions, reducing the ABA level in early embryos.
Over-expression of AhCYP707A4 in Nicotiana benthamiana
showed a phenotype of low ABA content with high numbers
of aborted embryos, small pods and less seeds, which confirms
that CYP707A4 is a key player in regulation of Ca2+ deficiency-induced
embryo abortion via ABA-mediated apoptosis. The
results elucidated the mechanism of low Ca2+-induced embryo
abortion. A set of varieties with different sizes of pods were evaluated
for their tolerance to calcium stress, and we found that
big pod varieties showed less tolerance to low calcium and more
easy to produce ill-filled pods or empty pods. Drought will make
worse of the phenomenon. Soil supplied with rich calcium will
improve the pods filling and greatly reduce number of empty
pods. We conclude big-pod varieties easier to produce empty
pods are also associated with calcium level in the soil.","2017-02","published",,,,,,,"P-056",,"InterDrought-V","Hyderabad, India","February 21-25, 2017","conference",,,,,,"TRUE",,,,,,,,"",,"http://idv.ceg.icrisat.org/wp-content/uploads/2017/02/Abstract_Book_Final.pdf","org",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"10711",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Zhang","C","","",,,,,,,,,,,,,,,,,,,,,,,"Fujian Provincial Key Laboratory of Crop Molecular and Cell Biology, Fujian Agriculture and Forestry University, Fuzhou (Fujian)","India",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"10711",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Cai","T C","","",,,,,,,,,,,,,,,,,,,,,,,"College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou (Fujian)",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"10711",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Deng","Y","","",,,,,,,,,,,,,,,,,,,,,,,"ICRISAT (Patancheru)",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"10711",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Zhou","S B","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"10711",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Varshney","R K","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"10711",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Zhuang","W J","","",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,