%0 Journal Article
%@ 2277-1808
%A Sailaja, B
%A Mangrauthia, S K
%A Voleti, S R
%A Subrahmanyam, D
%A Brajendra, -
%A Ravindra Babu, V
%D 2017
%F icrisat:10463
%I Academy for Environment and Life Sciences, India
%J Bulletin of Environment, Pharmacology and Life Sciences
%K Expression Analysis, Novel microRNAs, Rice, High Temperature Stress, Abiotic stress, N22, genes
%N 1
%P 225-229
%T Expression Analysis of Novel microRNAs in Rice During High  Temperature Stress
%U http://oar.icrisat.org/10463/
%V 6
%X MicroRNAs (miRNAs) are small non-coding RNAs which play an important role in regulating the genes involved in plant  growth and development. Several studies showed that miRNAs are involved in plants response to different kinds of  abiotic stresses also. In our previous study, temperature responsive miRNAs were predicted in O.sativa. 27 miRNAs were  predicted to be novel in rice using homology search. In continuation to our previous study, expression of 14 novel  miRNAs was done in shoot and root of 13 days old seedlings of five different rice cultivars using real time PCR. Expression  these miRNAs was analyzed in control and high temperature stress environment. Out of 14 predicted novel miRNAs, two  novel miRNAs- miR157a and miR165a showed expression in all five rice cultivars. Interestingly, miR165a showed a  differential expression pattern among heat tolerant (N22, IR64 and Rasi) and susceptible (Vandana and Sampada)  cultivars suggesting that it might have specific role in high temperature tolerance.
%Z Authors are highly thankful to the Director, Indian Institute of Rice Research, for his kind support.  Financial support received from NICRA (National Initiative of Climate and Resilient Agriculture) project is  acknowledged.