eprintid: 10460
rev_number: 11
eprint_status: archive
userid: 1305
dir: disk0/00/01/04/60
datestamp: 2018-03-03 09:58:30
lastmod: 2018-03-03 09:58:30
status_changed: 2018-03-03 09:58:30
type: article
metadata_visibility: show
creators_name: Durgaraju, C
creators_name: Srivastava, R K
creators_name: Reddy, N
creators_name: Katiyar, P
creators_name: Kilaru, K D
icrisatcreators_name: Srivastava, R K
icrisatcreators_name: Katiyar, P
affiliation: College of Agriculture, ANGRAU, Rajendranagar (Hyderabad)
affiliation: Seed Research and Technology Centre, PJTSAU, Rajendranagar (Hyderabad)
affiliation: ICRISAT (Patancheru)
affiliation: Department of Plant Pathology, College of Agriculture, Rajendranagar (Hyderabad)
country: India
title: Genetic Linkage Map Construction in Pearl Millet Using SSR Markers
ispublished: pub
subjects: PG2
subjects: S1.5.1
subjects: s2.13
divisions: CRPS3
full_text_status: public
keywords: Linkage Map; Polymerase Chain reaction; mapping population; pearl millet; Sclerospora graminicola; Downy mildew; Genotyping; SSR Markers ; Genetic Mapping
note: The first and second authors thank Acharya
N.G. Ranga Agricultural University
(ANGRAU) for financial help in the form of
stipend during the course of work.
abstract: For pearl millet production in the semi-arid tropics, Sclerospora graminicola is a major biotic
constraint causes Downy mildew. The pathogen is heterothallic and frequent recombination
leads to evolution of new virulent populations. Identification of resistance to new virulent isolates
is a prerequisite for resistance breeding. A very good understanding of ability to manipulate
oligogenic and quantitative traits is offered to plant breeders by recent advances in genetic
marker technology. In the present investigation genotypic data generated for a total of 88 marker
loci (39 Genomic SSRs and 49 EST SSRs) were used to construct a linkage map of the pearl
millet mapping population of 188 F8 RIL progenies based on the cross ICMB 89111-P6 x ICMB
90111-P6. A skeleton linkage map of seven linkage groups with a total map length of 725.5 cM
(Haldane units) was constructed using data from 74 marker loci for 188 RILs using JoinMap at
LOD threshold value of 5.0 and MapMaker/Exp version 3.0b and map was drawn using Map
Chart 2.2. The map length of individual linkage groups ranged from a minimum of 32.1cM (LG3)
to a maximum of 140.2 cM (LG1). The average inter marker distance was 9.8 cM, with an
average density of 0.102 markers/cM. The total number of mapped loci per linkage group (LG)
ranged from 5 on LG3 to 23 on LG1.
date: 2017
date_type: published
publication: International Journal of Pure & Applied Bioscience
volume: 5
number: 5
publisher: Vital Biotech, India
pagerange: 1304-1315
id_number: 10.18782/2320-7051.5663
refereed: TRUE
issn: 2320 – 7051
official_url: http://www.ijpab.com/vol5-iss5a186.php
related_url_url: https://scholar.google.co.in/scholar?hl=en&as_sdt=0%2C5&q=Genetic+Linkage+Map+Construction+in+Pearl+Millet+Using+SSR+Markers&btnG=
related_url_type: pub
citation:   Durgaraju, C and Srivastava, R K and Reddy, N and Katiyar, P and Kilaru, K D  (2017) Genetic Linkage Map Construction in Pearl Millet Using SSR Markers.  International Journal of Pure & Applied Bioscience, 5 (5).  pp. 1304-1315.  ISSN 2320 – 7051     
document_url: http://oar.icrisat.org/10460/1/Genetic%20Linkage%20Map%20Construction%20in%20Pearl%20Millet%20Using%20SSR%20Markers.pdf