<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Identification and validation of insertion–deletion polymorphisms in pigeonpea"^^ . "Completely sequenced plant genomes provide scope for designing\r\na large number of genome wide insertion–deletion (InDel)\r\nmarkers, which are useful in various aspects of crop breeding and\r\ngenetic analysis. With the objective of developing InDel markers\r\nfrom pigeonpea genome, the re-sequencing data of eight MAGIC\r\nparental lines were used to identify InDels using Dindel software.\r\nAs a result, a total of 102,181 InDels were identified. Of these\r\n70158 InDels were found unique. The higher number of InDels\r\nwere found in intergenic (43%) followed by upstream (26%)\r\nand downstream (24%) regions. A total of 6.93 % of Indels were\r\nfound in the genic region. Out of 70158 InDels, 2,426 (1032 Insertions\r\nand 1394 deletions) with ≥20 bp size among different\r\nparental lines were selected. Average distribution of selected\r\n2426 InDels was found 220 InDels/LG with maximum number\r\nof InDels on CcLG11 (385 InDels) and minimum number of In-\r\nDels on CcLG05 (70 InDels). A set of 293 InDels could assess\r\ngenetic diversity and establish phylogenetic relationships among\r\n16 parental lines of different mapping populations. Validation of\r\nthese primer pairs on parental lines of different mapping population\r\nresulted in higher amplification success rate (≥83%) with\r\nalmost 52.04% polymorphism rate among parental lines on 3%\r\nagarose gel. The number of alleles per locus ranged from 2 to\r\n9 with an average of 3.8 alleles. Further, to track the genome\r\nof parents in complex funnel crossing scheme of pigeonpea\r\nMAGIC population at 28-two way, 14-four way and 7-eigth way\r\nstages, we have identified unique InDel primers for each of the\r\n8 MAGIC parents. The result showed that InDel markers with\r\ntheir high polymorphic potential in comparison to SSR markers\r\nwould be preferred candidate markers in various marker-based\r\napplications in pigeonpea genetics and breeding."^^ . "2017-02" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "S"^^ . "Parupalli"^^ . "S Parupalli"^^ . . "S K"^^ . "Singh"^^ . "S K Singh"^^ . . "C V"^^ . "Sameer Kumar"^^ . "C V Sameer Kumar"^^ . . "R K"^^ . "Varshney"^^ . "R K Varshney"^^ . . "J"^^ . "Obala"^^ . "J Obala"^^ . . "P"^^ . "Sinha"^^ . "P Sinha"^^ . . "V"^^ . "Suryanaraya"^^ . "V Suryanaraya"^^ . . "S M"^^ . "Kale"^^ . "S M Kale"^^ . . "R K"^^ . "Saxena"^^ . "R K Saxena"^^ . . . . "InterDrought-V"^^ . . . . . "Hyderabad, India"^^ . . . . . . "Identification and validation of insertion–deletion polymorphisms in pigeonpea (PDF)"^^ . . . . . "Abstract_Book_423.pdf"^^ . . . "Identification and validation of insertion–deletion polymorphisms in pigeonpea (Other)"^^ . . . . . . "lightbox.jpg"^^ . . . "Identification and validation of insertion–deletion polymorphisms in pigeonpea (Other)"^^ . . . . . . "preview.jpg"^^ . . . "Identification and validation of insertion–deletion polymorphisms in pigeonpea (Other)"^^ . . . . . . "medium.jpg"^^ . . . "Identification and validation of insertion–deletion polymorphisms in pigeonpea (Other)"^^ . . . . . . "small.jpg"^^ . . . "Identification and validation of insertion–deletion polymorphisms in pigeonpea (Other)"^^ . . . . . . "indexcodes.txt"^^ . . "HTML Summary of #10315 \n\nIdentification and validation of insertion–deletion polymorphisms in pigeonpea\n\n" . "text/html" . . . "Pigeonpea"@en . . . "Genetics and Genomics"@en . .