eprintid: 10310
rev_number: 17
eprint_status: archive
userid: 1305
dir: disk0/00/01/03/10
datestamp: 2017-11-22 08:27:19
lastmod: 2018-03-04 12:48:54
status_changed: 2017-11-22 08:27:19
type: conference_item
metadata_visibility: show
creators_name: Pazhamala, L T
creators_name: Saxena, R K
creators_name: Purohit, S
creators_name: Bajaj, P
creators_name: Kumar, V
creators_name: Garg, V
creators_name: Srikanth, S
creators_name: Hingane, A J
creators_name: Kulshreshtha, A
creators_name: Krishnamurthy, L
creators_name: Sameer Kumar, C V
creators_name: Verdier, J
creators_name: Varshney, R K
icrisatcreators_name: Pazhamala, L T
icrisatcreators_name: Saxena, R K
icrisatcreators_name: Purohit, S
icrisatcreators_name: Bajaj, P
icrisatcreators_name: Kumar, V
icrisatcreators_name: Garg, V
icrisatcreators_name: Hingane, A J
icrisatcreators_name: Kulshreshtha, A
icrisatcreators_name: Krishnamurthy, L
icrisatcreators_name: Sameer Kumar, C V
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
affiliation: Nanyang Technological University (Singapore)
affiliation: INRA - Research Institute in Horticulture and Seeds (IRHS) (Beaucouze)
affiliation: School of Plant Biology and Institute of Agriculture, The University of Western Australia (Crawley)
country: India
country: Singapore
country: France
country: Australia
title: Next generation sequencing based transcriptomic studies for crop improvement in pigeonpea
ispublished: pub
subjects: s1.2
subjects: s2.13
divisions: CRPS2
divisions: CRPS3
full_text_status: public
pres_type: paper
keywords: Next generation sequencing, Crop improvement, Pigeonpea, Transcriptomic studies
abstract: Transcriptomic studies are rapidly evolving as a powerful tool
with next-generation sequencing technology to understand gene
functions and molecular mechanisms. RNA sequencing (RNAseq)
provides a dynamic range for transcript detection and a
better quantification of expression levels.With the availability of
genome sequence in pigeonpea, RNA-seq was used to link the
sequence information to phenotypic traits resulting from specific
developmental processes. In pigeonpea, three-line hybrid
breeding system is well-established; however, it is technically
demanding and cumbersome. In order to explore the possibility
of a two-line hybrid breeding system, a coherent transcriptomic
approach supported by physiological and cytological data has
led to the identification of a temperature-sensitive male sterile
(TSMS) line. This line has been characterized for critical (tetrad)
stage and temperature (23°C), and the identification of candidate
genes involved in abscisic acid signaling for fertility reversion.
Furthermore, a gene expression atlas (CcGEA) has been developed
and transcriptomic profiles generated for studying pod
and seed development with a dataset of 590.84 and 342 million
paired-end reads, respectively in pigeonpea. These data have
been analyzed for genes with differential, specific, spatio-temporal
and constitutive expression. In addition, CcGEA identified a
gene network of 28 co-expressed genes, including two regulatory
genes, a pollen specific SF3 and a sucrose-proton symporter to
be involved in pollen fertility, which has potential implication in
seed yield improvement. In summary, this study, especially identification
of TSMS and development of CcGEA, will accelerate
on-going efforts to enhance genetic gains in pigeonpea.
date: 2017-02
date_type: published
pagerange: 220
event_title: InterDrought-V
event_location: Hyderabad, India
event_dates: February 21-25, 2017
event_type: conference
refereed: TRUE
related_url_url: http://idv.ceg.icrisat.org/wp-content/uploads/2017/02/Abstract_Book_Final.pdf
related_url_type: org
citation:   Pazhamala, L T and Saxena, R K and Purohit, S and Bajaj, P and Kumar, V and Garg, V and Srikanth, S and Hingane, A J and Kulshreshtha, A and Krishnamurthy, L and Sameer Kumar, C V and Verdier, J and Varshney, R K  (2017) Next generation sequencing based transcriptomic studies for crop improvement in pigeonpea.  In: InterDrought-V, February 21-25, 2017, Hyderabad, India.     
document_url: http://oar.icrisat.org/10310/1/Abstract_Book_412.pdf