eprintid: 10304
rev_number: 17
eprint_status: archive
userid: 1305
dir: disk0/00/01/03/04
datestamp: 2017-11-22 05:40:32
lastmod: 2018-07-03 09:05:57
status_changed: 2017-11-22 05:40:32
type: conference_item
metadata_visibility: show
creators_name: Kale, S M
creators_name: Jaganathan, D
creators_name: Roorkiwal, M
creators_name: Thudi, M
creators_name: Purushothaman, R
creators_name: Praveenkumar, M
creators_name: Krishnamurthy, L
creators_name: Kavi Kishor, P B
creators_name: Gaur, P M
creators_name: Varshney, R K
icrisatcreators_name: Kale, S M
icrisatcreators_name: Jaganathan, D
icrisatcreators_name: Roorkiwal, M
icrisatcreators_name: Thudi, M
icrisatcreators_name: Purushothaman, R
icrisatcreators_name: Praveenkumar, M
icrisatcreators_name: Krishnamurthy, L
icrisatcreators_name: Gaur, P M
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
affiliation: Osmania University (Hyderabad)
affiliation: The University of Western Australia (Crawley)
country: India
country: Australia
title: Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed
weight and drought related traits in chickpea
ispublished: pub
subjects: ABio
subjects: DT1
subjects: s1.1
subjects: s1000
subjects: s2.13
divisions: CRPS3
full_text_status: public
pres_type: poster
keywords: Fine mapping studies, QTL-hotspot, Drought related traits, Chickpea
abstract: A “QTL-hotspot” region of 7 Mb size for drought component traits
was identified on CaLG04, using a recombinant inbred line (RIL)
population (ICC 4958 × ICC 1882) in chickpea. Further, skimbased
genotyping by sequencing (GBS) approach with large SNP
markers delimited the “QTL-hotspot” region into two sub-regions;
viz, “QTL-hotspot_a” of 139.22 kb and “QTL-hotspot_b” of 153.36
kb, on the kabuli draft genome sequence. In order to validate
and identify more recombinations in the sub-regions for further
refinement, a fine mapping population with 1,911 lines was developed.
Flanking markers of the two “QTL-hotspot” sub-regions
were converted to KASPar assays and used to screen the fine
mapping population consisting of 1,911 lines. As a result, 19 F2:3
recombinant families were identified. These families were phenotyped
for seed weight and other drought-related traits. Comparison
of genotype and phenotype data identified a genomic region
of ~113 Kb size within “QTL-hotspot_a” responsible for 100 seed
weight (100SDW) and other drought-related traits in chickpea.
Subsequently, a syntenic study between the refined “QTL-hotpsot”
region and desi genome identified a 2 Mb region on Ca_LG_4
pseudomolecule. Whole genome re-sequencing (WGRS) analysis
of selected lines from each recombinant family identified
several non-synonymous and InDel mutations within important
candidate genes. Functional validation of these genes will help
decipher the mechanism of drought stress tolerance in chickpea.
date: 2017-02
date_type: published
pagerange: 210
event_title: InterDrought-V
event_location: Hyderabad, India
event_dates: February 21-25, 2017
event_type: conference
refereed: TRUE
related_url_url: http://idv.ceg.icrisat.org/wp-content/uploads/2017/02/Abstract_Book_Final.pdf
related_url_type: org
citation:   Kale, S M and Jaganathan, D and Roorkiwal, M and Thudi, M and Purushothaman, R and Praveenkumar, M and Krishnamurthy, L and Kavi Kishor, P B and Gaur, P M and Varshney, R K  (2017) Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed weight and drought related traits in chickpea.  In: InterDrought-V, February 21-25, 2017, Hyderabad, India.     
document_url: http://oar.icrisat.org/10304/1/Abstract_Book_402.pdf