TY  - CONF
N2  - A ?QTL-hotspot? region of 7 Mb size for drought component traits
was identified on CaLG04, using a recombinant inbred line (RIL)
population (ICC 4958 × ICC 1882) in chickpea. Further, skimbased
genotyping by sequencing (GBS) approach with large SNP
markers delimited the ?QTL-hotspot? region into two sub-regions;
viz, ?QTL-hotspot_a? of 139.22 kb and ?QTL-hotspot_b? of 153.36
kb, on the kabuli draft genome sequence. In order to validate
and identify more recombinations in the sub-regions for further
refinement, a fine mapping population with 1,911 lines was developed.
Flanking markers of the two ?QTL-hotspot? sub-regions
were converted to KASPar assays and used to screen the fine
mapping population consisting of 1,911 lines. As a result, 19 F2:3
recombinant families were identified. These families were phenotyped
for seed weight and other drought-related traits. Comparison
of genotype and phenotype data identified a genomic region
of ~113 Kb size within ?QTL-hotspot_a? responsible for 100 seed
weight (100SDW) and other drought-related traits in chickpea.
Subsequently, a syntenic study between the refined ?QTL-hotpsot?
region and desi genome identified a 2 Mb region on Ca_LG_4
pseudomolecule. Whole genome re-sequencing (WGRS) analysis
of selected lines from each recombinant family identified
several non-synonymous and InDel mutations within important
candidate genes. Functional validation of these genes will help
decipher the mechanism of drought stress tolerance in chickpea.
AV  - public
KW  - Fine mapping studies
KW  -  QTL-hotspot
KW  -  Drought related traits
KW  -  Chickpea
A1  - Kale, S M
A1  - Jaganathan, D
A1  - Roorkiwal, M
A1  - Thudi, M
A1  - Purushothaman, R
A1  - Praveenkumar, M
A1  - Krishnamurthy, L
A1  - Kavi Kishor, P B
A1  - Gaur, P M
A1  - Varshney, R K
TI  - Fine mapping studies identified a 113 kb region within ?QTL-hotspot_a? for seed
weight and drought related traits in chickpea
UR  - http://oar.icrisat.org/10304/
T2  - InterDrought-V
Y1  - 2017/02//
ID  - icrisat10304
M2  - Hyderabad, India
ER  -