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dc:title "HTML Summary of #10304 \n\nFine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed
\nweight and drought related traits in chickpea\n\n";
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bibo:abstract "A “QTL-hotspot” region of 7 Mb size for drought component traits\r\nwas identified on CaLG04, using a recombinant inbred line (RIL)\r\npopulation (ICC 4958 × ICC 1882) in chickpea. Further, skimbased\r\ngenotyping by sequencing (GBS) approach with large SNP\r\nmarkers delimited the “QTL-hotspot” region into two sub-regions;\r\nviz, “QTL-hotspot_a” of 139.22 kb and “QTL-hotspot_b” of 153.36\r\nkb, on the kabuli draft genome sequence. In order to validate\r\nand identify more recombinations in the sub-regions for further\r\nrefinement, a fine mapping population with 1,911 lines was developed.\r\nFlanking markers of the two “QTL-hotspot” sub-regions\r\nwere converted to KASPar assays and used to screen the fine\r\nmapping population consisting of 1,911 lines. As a result, 19 F2:3\r\nrecombinant families were identified. These families were phenotyped\r\nfor seed weight and other drought-related traits. Comparison\r\nof genotype and phenotype data identified a genomic region\r\nof ~113 Kb size within “QTL-hotspot_a” responsible for 100 seed\r\nweight (100SDW) and other drought-related traits in chickpea.\r\nSubsequently, a syntenic study between the refined “QTL-hotpsot”\r\nregion and desi genome identified a 2 Mb region on Ca_LG_4\r\npseudomolecule. Whole genome re-sequencing (WGRS) analysis\r\nof selected lines from each recombinant family identified\r\nseveral non-synonymous and InDel mutations within important\r\ncandidate genes. Functional validation of these genes will help\r\ndecipher the mechanism of drought stress tolerance in chickpea."^^xsd:string;
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skos:prefLabel "Abiotic Stress"@en .
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skos:prefLabel "Drought Tolerance"@en .
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skos:prefLabel "Chickpea"@en .
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