<mods:mods version="3.3" xsi:schemaLocation="http://www.loc.gov/mods/v3 http://www.loc.gov/standards/mods/v3/mods-3-3.xsd" xmlns:mods="http://www.loc.gov/mods/v3" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"><mods:titleInfo><mods:title>Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed&#13;
weight and drought related traits in chickpea</mods:title></mods:titleInfo><mods:name type="personal"><mods:namePart type="given">S M</mods:namePart><mods:namePart type="family">Kale</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">D</mods:namePart><mods:namePart type="family">Jaganathan</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">M</mods:namePart><mods:namePart type="family">Roorkiwal</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">M</mods:namePart><mods:namePart type="family">Thudi</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">R</mods:namePart><mods:namePart type="family">Purushothaman</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">M</mods:namePart><mods:namePart type="family">Praveenkumar</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">L</mods:namePart><mods:namePart type="family">Krishnamurthy</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">P B</mods:namePart><mods:namePart type="family">Kavi Kishor</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">P M</mods:namePart><mods:namePart type="family">Gaur</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:name type="personal"><mods:namePart type="given">R K</mods:namePart><mods:namePart type="family">Varshney</mods:namePart><mods:role><mods:roleTerm type="text">author</mods:roleTerm></mods:role></mods:name><mods:abstract>A “QTL-hotspot” region of 7 Mb size for drought component traits&#13;
was identified on CaLG04, using a recombinant inbred line (RIL)&#13;
population (ICC 4958 × ICC 1882) in chickpea. Further, skimbased&#13;
genotyping by sequencing (GBS) approach with large SNP&#13;
markers delimited the “QTL-hotspot” region into two sub-regions;&#13;
viz, “QTL-hotspot_a” of 139.22 kb and “QTL-hotspot_b” of 153.36&#13;
kb, on the kabuli draft genome sequence. In order to validate&#13;
and identify more recombinations in the sub-regions for further&#13;
refinement, a fine mapping population with 1,911 lines was developed.&#13;
Flanking markers of the two “QTL-hotspot” sub-regions&#13;
were converted to KASPar assays and used to screen the fine&#13;
mapping population consisting of 1,911 lines. As a result, 19 F2:3&#13;
recombinant families were identified. These families were phenotyped&#13;
for seed weight and other drought-related traits. Comparison&#13;
of genotype and phenotype data identified a genomic region&#13;
of ~113 Kb size within “QTL-hotspot_a” responsible for 100 seed&#13;
weight (100SDW) and other drought-related traits in chickpea.&#13;
Subsequently, a syntenic study between the refined “QTL-hotpsot”&#13;
region and desi genome identified a 2 Mb region on Ca_LG_4&#13;
pseudomolecule. Whole genome re-sequencing (WGRS) analysis&#13;
of selected lines from each recombinant family identified&#13;
several non-synonymous and InDel mutations within important&#13;
candidate genes. Functional validation of these genes will help&#13;
decipher the mechanism of drought stress tolerance in chickpea.</mods:abstract><mods:classification authority="lcc">Abiotic Stress</mods:classification><mods:classification authority="lcc">Drought Tolerance</mods:classification><mods:classification authority="lcc">Chickpea</mods:classification><mods:classification authority="lcc">Drought</mods:classification><mods:classification authority="lcc">Genetics and Genomics</mods:classification><mods:originInfo><mods:dateIssued encoding="iso8061">2017-02</mods:dateIssued></mods:originInfo><mods:genre>Conference or Workshop Item</mods:genre></mods:mods>