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        <dc:title>Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed&#13;
weight and drought related traits in chickpea</dc:title>
        <dc:creator>Kale, S M</dc:creator>
        <dc:creator>Jaganathan, D</dc:creator>
        <dc:creator>Roorkiwal, M</dc:creator>
        <dc:creator>Thudi, M</dc:creator>
        <dc:creator>Purushothaman, R</dc:creator>
        <dc:creator>Praveenkumar, M</dc:creator>
        <dc:creator>Krishnamurthy, L</dc:creator>
        <dc:creator>Kavi Kishor, P B</dc:creator>
        <dc:creator>Gaur, P M</dc:creator>
        <dc:creator>Varshney, R K</dc:creator>
        <dc:subject>Abiotic Stress</dc:subject>
        <dc:subject>Drought Tolerance</dc:subject>
        <dc:subject>Chickpea</dc:subject>
        <dc:subject>Drought</dc:subject>
        <dc:subject>Genetics and Genomics</dc:subject>
        <dc:description>A “QTL-hotspot” region of 7 Mb size for drought component traits&#13;
was identified on CaLG04, using a recombinant inbred line (RIL)&#13;
population (ICC 4958 × ICC 1882) in chickpea. Further, skimbased&#13;
genotyping by sequencing (GBS) approach with large SNP&#13;
markers delimited the “QTL-hotspot” region into two sub-regions;&#13;
viz, “QTL-hotspot_a” of 139.22 kb and “QTL-hotspot_b” of 153.36&#13;
kb, on the kabuli draft genome sequence. In order to validate&#13;
and identify more recombinations in the sub-regions for further&#13;
refinement, a fine mapping population with 1,911 lines was developed.&#13;
Flanking markers of the two “QTL-hotspot” sub-regions&#13;
were converted to KASPar assays and used to screen the fine&#13;
mapping population consisting of 1,911 lines. As a result, 19 F2:3&#13;
recombinant families were identified. These families were phenotyped&#13;
for seed weight and other drought-related traits. Comparison&#13;
of genotype and phenotype data identified a genomic region&#13;
of ~113 Kb size within “QTL-hotspot_a” responsible for 100 seed&#13;
weight (100SDW) and other drought-related traits in chickpea.&#13;
Subsequently, a syntenic study between the refined “QTL-hotpsot”&#13;
region and desi genome identified a 2 Mb region on Ca_LG_4&#13;
pseudomolecule. Whole genome re-sequencing (WGRS) analysis&#13;
of selected lines from each recombinant family identified&#13;
several non-synonymous and InDel mutations within important&#13;
candidate genes. Functional validation of these genes will help&#13;
decipher the mechanism of drought stress tolerance in chickpea.</dc:description>
        <dc:date>2017-02</dc:date>
        <dc:type>Conference or Workshop Item</dc:type>
        <dc:type>PeerReviewed</dc:type>
        <dc:format>application/pdf</dc:format>
        <dc:language>en</dc:language>
        <dc:identifier>http://oar.icrisat.org/10304/1/Abstract_Book_402.pdf</dc:identifier>
        <dc:identifier>  Kale, S M and Jaganathan, D and Roorkiwal, M and Thudi, M and Purushothaman, R and Praveenkumar, M and Krishnamurthy, L and Kavi Kishor, P B and Gaur, P M and Varshney, R K  (2017) Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed weight and drought related traits in chickpea.  In: InterDrought-V, February 21-25, 2017, Hyderabad, India.     </dc:identifier></oai_dc:dc>
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