relation: http://oar.icrisat.org/10304/
title: Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed  weight and drought related traits in chickpea
creator: Kale, S M
creator: Jaganathan, D
creator: Roorkiwal, M
creator: Thudi, M
creator: Purushothaman, R
creator: Praveenkumar, M
creator: Krishnamurthy, L
creator: Kavi Kishor, P B
creator: Gaur, P M
creator: Varshney, R K
subject: Abiotic Stress
subject: Drought Tolerance
subject: Chickpea
subject: Drought
subject: Genetics and Genomics
description: A “QTL-hotspot” region of 7 Mb size for drought component traits  was identified on CaLG04, using a recombinant inbred line (RIL)  population (ICC 4958 × ICC 1882) in chickpea. Further, skimbased  genotyping by sequencing (GBS) approach with large SNP  markers delimited the “QTL-hotspot” region into two sub-regions;  viz, “QTL-hotspot_a” of 139.22 kb and “QTL-hotspot_b” of 153.36  kb, on the kabuli draft genome sequence. In order to validate  and identify more recombinations in the sub-regions for further  refinement, a fine mapping population with 1,911 lines was developed.  Flanking markers of the two “QTL-hotspot” sub-regions  were converted to KASPar assays and used to screen the fine  mapping population consisting of 1,911 lines. As a result, 19 F2:3  recombinant families were identified. These families were phenotyped  for seed weight and other drought-related traits. Comparison  of genotype and phenotype data identified a genomic region  of ~113 Kb size within “QTL-hotspot_a” responsible for 100 seed  weight (100SDW) and other drought-related traits in chickpea.  Subsequently, a syntenic study between the refined “QTL-hotpsot”  region and desi genome identified a 2 Mb region on Ca_LG_4  pseudomolecule. Whole genome re-sequencing (WGRS) analysis  of selected lines from each recombinant family identified  several non-synonymous and InDel mutations within important  candidate genes. Functional validation of these genes will help  decipher the mechanism of drought stress tolerance in chickpea.
date: 2017-02
type: Conference or Workshop Item
type: PeerReviewed
format: application/pdf
language: en
identifier: http://oar.icrisat.org/10304/1/Abstract_Book_402.pdf
identifier:   Kale, S M and Jaganathan, D and Roorkiwal, M and Thudi, M and Purushothaman, R and Praveenkumar, M and Krishnamurthy, L and Kavi Kishor, P B and Gaur, P M and Varshney, R K  (2017) Fine mapping studies identified a 113 kb region within “QTL-hotspot_a” for seed weight and drought related traits in chickpea.  In: InterDrought-V, February 21-25, 2017, Hyderabad, India.