@inproceedings{icrisat10304,
           month = {February},
       booktitle = {InterDrought-V},
            year = {2017},
           title = {Fine mapping studies identified a 113 kb region within ?QTL-hotspot\_a? for seed
weight and drought related traits in chickpea},
           pages = {210},
          author = {S M Kale and D Jaganathan and M Roorkiwal and M Thudi and R Purushothaman and M Praveenkumar and L Krishnamurthy and P B Kavi Kishor and P M Gaur and R K Varshney},
        keywords = {Fine mapping studies, QTL-hotspot, Drought related traits, Chickpea},
             url = {http://oar.icrisat.org/10304/},
        abstract = {A ?QTL-hotspot? region of 7 Mb size for drought component traits
was identified on CaLG04, using a recombinant inbred line (RIL)
population (ICC 4958 {$\times$} ICC 1882) in chickpea. Further, skimbased
genotyping by sequencing (GBS) approach with large SNP
markers delimited the ?QTL-hotspot? region into two sub-regions;
viz, ?QTL-hotspot\_a? of 139.22 kb and ?QTL-hotspot\_b? of 153.36
kb, on the kabuli draft genome sequence. In order to validate
and identify more recombinations in the sub-regions for further
refinement, a fine mapping population with 1,911 lines was developed.
Flanking markers of the two ?QTL-hotspot? sub-regions
were converted to KASPar assays and used to screen the fine
mapping population consisting of 1,911 lines. As a result, 19 F2:3
recombinant families were identified. These families were phenotyped
for seed weight and other drought-related traits. Comparison
of genotype and phenotype data identified a genomic region
of {\texttt{\char126}}113 Kb size within ?QTL-hotspot\_a? responsible for 100 seed
weight (100SDW) and other drought-related traits in chickpea.
Subsequently, a syntenic study between the refined ?QTL-hotpsot?
region and desi genome identified a 2 Mb region on Ca\_LG\_4
pseudomolecule. Whole genome re-sequencing (WGRS) analysis
of selected lines from each recombinant family identified
several non-synonymous and InDel mutations within important
candidate genes. Functional validation of these genes will help
decipher the mechanism of drought stress tolerance in chickpea.}
}