"10283","8","archive","1305",,,"disk0/00/01/02/83","2017-11-16 10:46:05","2017-11-16 10:46:05","2017-11-16 10:46:05","article",,,"show","Library-ICRISAT@CGIAR.ORG",,,"","","","","","","","","","",,,,"Kaushal","M","","","","","","","Kaushal","M","","","","",,,,,"","",,,,,"","","ICRISAT (Patancheru)","India","Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum","pub","s2.4","CRPS2","","restricted",,,"Bacillus pumilus, Sclerotinia sclerotiorum, 16S rRNA, Disease suppression, Cauliflower production",,"Authors thanks to ICAR, New Delhi for financial
support provided to carry out this study.","A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35 °C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus. Furthermore, the genome of this isolate was searched for antimicrobial lipopeptide, i.e., ItuD and SrfC genes. The PCR amplification results showed the presence of both these lipopeptide genes in isolate YSPMK11. Iturin A as antifungal compound was identified as major components of fraction through GC/MS. In field experiments, the application of strain YSPMK11 cell suspension (108 CFU/ml) suppressed disease severity by 93% and increased curd yield by 36% which was more that of commercially used fungicide in farmer practices under mid-hills of Himachal Pradesh. Conclusively, our study is first to demonstrate the effect of B. pumilus strain YSPMK11 in suppression of SSR under field conditions and would be employed as an efficient biocontrol agent to replace commercial fungicides in cauliflower cropping system. In addition, the presence of both lipopeptide genes (ItuD and SrfC) and iturin A in this isolate makes him potent strain for biological control application in agriculture.","2017-06","published",,"3 Biotech","7 (2)","90","Springer",,"1-9",,,,,,"10.1007/s13205-017-0732-7",,,,,"TRUE",,"2190-572X",,,,,,"","http://dx.doi.org/10.1007/s13205-017-0732-7","https://scholar.google.co.in/scholar?hl=en&as_sdt=0%2C5&q=Bacillus+pumilus+strain+YSPMK11+as+plant+growth+promoter+and+bicontrol+agent+against+Sclerotinia+sclerotiorum&btnG=","pub",,"ICAR","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
"10283",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Kumar","A","","",,,,,,,,,,,,,,,,,,,,,,,"Department of Biological Sciences, Faculty of Agriculture, Science and Technology, School of Environmental and Health Sciences, North-West University (Potchefstroom)","South Africa",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
"10283",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,"Kaushal","R","","",,,,,,,,,,,,,,,,,,,,,,,"Department of Basic Science, Dr. Y. S. Parmar University of Horticulture and Forestry (Solan)",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,