<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Identification and characterization of herbicide\r\ntolerant mutant lines using SNP marker(s) in\r\nchickpea (Cicer arietinum L.)"^^ . "Chickpea is a major food legume and an important source of protein in many countries\r\nof south Asia and sub-Saharan African. Globally, chickpea is cultivated over an area of 13.9\r\nmillion hectares, with the production of 13.7 million tons. Besides a number of biotic and\r\nabiotic stresses that lead to significant yield losses in chickpea, weeds are also reported to\r\nreduce yield up to 84%, and severe yield losses as high as 98% are reported in autumn-sown\r\nchickpea. Hand weeding and mechanical weed control methods traditionally followed are\r\nbecoming expensive owing to increased cost of human labour. Chickpea cultivars with\r\nherbicide tolerance can serve as an alternative to this problem. Hence, it is essential to identify\r\nsources of herbicide tolerance and utilize them in developing herbicide tolerant cultivars.\r\nDevelopment of chickpea cultivars with herbicide resistance is considered to be an\r\neconomic and effective way for weed control. Earlier studies have reported large genetic\r\nvariation existing in chickpea germplasm for Imidazolinone (IMI) herbicide tolerance.IMI\r\ngroup of herbicides are considered as powerful means of weed control, and have many\r\nagronomic advantages. Imidazolinones are protein synthesis inhibitors and act by inhibiting\r\nthe enzyme acetohydroxyacid synthase (AHAS, also known as acetolactate synthase, ALS),\r\nwhich is a critical enzyme in the biosynthsis pathway of branched chain amino acids.\r\nA point mutation in the chickpea AHAS gene at Cytocine675 to Thymin675 confers\r\nresistance to imidazolinones. Thompson and Taran (2014) developed an allele-specific SNP\r\n(KASPar) marker using this point mutation to predict the phenotypic response of the\r\ngenotypes to IMI herbicides. This KASPar marker was used to genotype set of forty EMS\r\nmutant lines (developed in the background of JG 11 and KAK 2) and eighty four breeding\r\nlines in this study. In total of 124 genotypes along with check varieties (JG 11 and KAK 2)\r\nwere phenotyped for herbicide resistance under field conditions at ICRISAT, Patancheru\r\nduring Rabi, 2016. Herbicide tolerance ratings based on plant injury on a 1-5 scale (Gaur et\r\nal., 2013) was used for phenotyping. All the genotypes exhibited plant injury symptoms under\r\nIMI herbicide treatment. Among 126 genotypes, 8 were highly susceptible, 24 were\r\nmoderately tolerant and others were susceptible. The highly susceptible lines had 80-100%\r\nmortality. The genotypes which survived put forth secondary growth after 20-25 days of\r\nherbicide application leading to flowering and pod set. Upon genotyping with the KASPar\r\nmarker 124 out of 126 genotypes yielded the fluorescent data. Graphical visualization of the SNP genotyping data (KlusterCaller software) showed all the genotypes forming a single\r\ncluster near to allele ‘C’, associated with IMI susceptibility.\r\nIn addition to KASPar genotyping, an attempt was made to find the possibility of\r\nother allelic variation associated with herbicide tolerance. The AHAS gene sequence was\r\nblasted in the chickpea reference genome, and the best hit was used as query sequence to find\r\nSNP candidates from the available resequencing data of chickpea genotypes. Among all the\r\nvariations obtained, one SNP showing consistent variation was selected and converted to\r\nCAPS marker. Forty randomly selected genotypes belonging to different phenotypic classes\r\nwere analysed using the CAPS marker. All the genotypes exhibited similar banding pattern\r\nshowing no variation at the locus.\r\nThe KASPar marker reported by Thompson and Taran does not serve as a diagnostic\r\ntool in identification of herbicide tolerance. As none of the genotypes used in the study were\r\nhighly resistant, further screening of a large set of germplasm lines for herbicide tolerance and\r\namplicon sequencing of the AHAS gene in these lines will help in the identification of\r\nalternate alleles and the development of diagnostic marker for herbicide resistance."^^ . "2017" . . . "Professor Jayashankar Telangana State Agriculture University"^^ . . . "Department of Genetics and Plant Breeding, Professor Jayashankar Telangana State Agriculture University"^^ . . . . . . . . "A.M."^^ . "Teggi"^^ . "A.M. Teggi"^^ . . . . . . "Identification and characterization of herbicide\r\ntolerant mutant lines using SNP marker(s) in\r\nchickpea (Cicer arietinum L.) (PDF)"^^ . . . . . "Identification and characterization of herbicide\r\ntolerant mutant lines using SNP marker(s) in\r\nchickpea (Cicer arietinum L.) (Other)"^^ . . . . . . "Identification and characterization of herbicide\r\ntolerant mutant lines using SNP marker(s) in\r\nchickpea (Cicer arietinum L.) (Other)"^^ . . . . . . "Identification and characterization of herbicide\r\ntolerant mutant lines using SNP marker(s) in\r\nchickpea (Cicer arietinum L.) (Other)"^^ . . . . . . "Identification and characterization of herbicide\r\ntolerant mutant lines using SNP marker(s) in\r\nchickpea (Cicer arietinum L.) (Other)"^^ . . . . . . "Identification and characterization of herbicide\r\ntolerant mutant lines using SNP marker(s) in\r\nchickpea (Cicer arietinum L.) (Other)"^^ . . . . . "HTML Summary of #10264 \n\nIdentification and characterization of herbicide \ntolerant mutant lines using SNP marker(s) in \nchickpea (Cicer arietinum L.)\n\n" . "text/html" . . . "Chickpea"@en . .