eprintid: 10239
rev_number: 16
eprint_status: archive
userid: 1305
dir: disk0/00/01/02/39
datestamp: 2017-10-31 05:58:32
lastmod: 2018-03-08 04:47:22
status_changed: 2017-10-31 05:58:32
type: article
metadata_visibility: show
creators_name: Ramakrishnan, M
creators_name: Ceasar, S A
creators_name: Vinod, K K
creators_name: Duraipandiyan, V
creators_name: Ajeesh Krishna, T P
creators_name: Upadhyaya, H D
creators_name: Al-Dhabi, N A
creators_name: Ignacimuthu, S
icrisatcreators_name: Upadhyaya, H D
affiliation: Division of Plant Biotechnology, Entomology Research Institute, Loyola College (Chennai)
affiliation: Centre for Plant Sciences and School of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds (Leeds)
affiliation: ICAR-Indian Agricultural Research Institute, Rice Breeding and Genetics Research Centre (Aduthurai)
affiliation: Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University (Riyadh)
affiliation: ICRISAT (Patancheru)
affiliation: The International Scientific Partnership Program (ISPP), King Saud University, Vice-19 Rectorate for Graduate studies and Research (Riyadh)
country: India
country: UK
country: Saudi Arabia
title: Identification of putative QTLs for seedling stage phosphorus starvation response in finger millet (Eleusine coracana L. Gaertn.) by association mapping and cross species synteny analysis
ispublished: pub
subjects: S1.5
subjects: S1.5.2
subjects: s2.13
subjects: s3000
divisions: CRPS3
full_text_status: public
keywords: Finger millet, Germplasms, QTLs, Phosphorus, Small millets, Cereals, Association mapping, Genotypes, Millet germplasm
note: The authors thank the Department of Biotechnology, Government of India, New Delhi, for providing financial support through a grant BT/PR15011/AGR/02/772/2010. They also extend their appreciation to the International Scientific Partnership Program (ISPP) at King Saud University for support of this research work through ISPP 0020. Authors acknowledge the help rendered by ICRISAT, UAS (GKVK) and TNAU by providing finger millet germplasm. We thank Dr. Ketrien Devos, the University of Georgia, College of Agricultural Science, USA for giving the parent sequences of the SSR markers. We thank the mentor Dr P. Kadirvel, Senior Scientist, DOR, ICAR, Hyderabad-500030-India, for his encouragement.
abstract: A germplasm assembly of 128 finger millet genotypes from 18 countries was evaluated for seedling-stage phosphorus (P) responses by growing them in P sufficient (Psuf) and P deficient (Pdef) treatments. Majority of the genotypes showed adaptive responses to low P condition. Based on phenotype behaviour using the best linear unbiased predictors for each trait, genotypes were classified into, P responsive, low P tolerant and P non-responsive types. Based on the overall phenotype performance under Pdef, 10 genotypes were identified as low P tolerants. The low P tolerant genotypes were characterised by increased shoot and root length and increased root hair induction with longer root hairs under Pdef, than under Psuf. Association mapping of P response traits using mixed linear models revealed four quantitative trait loci (QTLs). Two QTLs (qLRDW.1 and qLRDW.2) for low P response affecting root dry weight explained over 10% phenotypic variation. In silico synteny analysis across grass genomes for these QTLs identified putative candidate genes such as Ser-Thr kinase and transcription factors such as WRKY and basic helix-loop-helix (bHLH). The QTLs for response under Psuf were mapped for traits such as shoot dry weight (qHSDW.1) and root length (qHRL.1). Putative associations of these QTLs over the syntenous regions on the grass genomes revealed proximity to cytochrome P450, phosphate transporter and pectin methylesterase inhibitor (PMEI) genes. This is the first report of the extent of phenotypic variability for P response in finger millet genotypes during seedling-stage, along with the QTLs and putative candidate genes associated with P starvation tolerance.
date: 2017-08-18
date_type: published
publication: PLoS ONE
publisher: Public Library of Science
pagerange: 1-27
id_number: 10.1371/journal.pone.0183261
refereed: TRUE
issn: 1932-6203
official_url: http://dx.doi.org/10.1371/journal.pone.0183261
related_url_url: https://scholar.google.co.in/scholar?hl=en&as_sdt=0%2C5&q=%3A+Identification+of+putative+QTLs+for+seedling+stage+phosphorus+starvation+response+in+finger+millet+%28Eleusine+coracana+L.+Gaertn.%29+by+association+mapping+and+cross+species+synteny+analysis&b
related_url_type: pub
citation:   Ramakrishnan, M and Ceasar, S A and Vinod, K K and Duraipandiyan, V and Ajeesh Krishna, T P and Upadhyaya, H D and Al-Dhabi, N A and Ignacimuthu, S  (2017) Identification of putative QTLs for seedling stage phosphorus starvation response in finger millet (Eleusine coracana L. Gaertn.) by association mapping and cross species synteny analysis.  PLoS ONE.  pp. 1-27.  ISSN 1932-6203     
document_url: http://oar.icrisat.org/10239/1/journal.pone.0183261.pdf