eprintid: 10234
rev_number: 11
eprint_status: archive
userid: 1305
dir: disk0/00/01/02/34
datestamp: 2017-10-26 05:17:29
lastmod: 2017-10-26 05:17:29
status_changed: 2017-10-26 05:17:29
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Reddy, P S
creators_name: Tharanya, M
creators_name: Sivasakthi, K
creators_name: Srikanth, M
creators_name: Hash, C T
creators_name: Kholova, J
creators_name: Sharma, K K
creators_name: Vadez, V
icrisatcreators_name: Reddy, P S
icrisatcreators_name: Tharanya, M
icrisatcreators_name: Sivasakthi, K
icrisatcreators_name: Srikanth, M
icrisatcreators_name: Hash, C T
icrisatcreators_name: Kholova, J
icrisatcreators_name: Sharma, K K
icrisatcreators_name: Vadez, V
affiliation: ICRISAT (Patancheru)
affiliation: Department of Industrial Biotechnology, Bharathidasan University (Tiruchirappalli)
country: India
title: Molecular cloning and expression analysis of Aquaporin genes in pearl millet [ Pennisetum glaucum (L) R. Br.] genotypes contrasting in their transpiration response to high vapour pressure deficits
ispublished: pub
subjects: MB1
subjects: S1.5.1
subjects: s2.13
divisions: CRPS3
crps: crp1.4
full_text_status: restricted
keywords: Pearl millet, Molecular cloning, Aquaporins, Transpiration rate, Vapour pressure deficit, VPD-sensitive genotype, VPD-insensitive genotype, Drought tolerance
note: PSR is thankful to the Department of Science and Technology, Govt.
of India, New Delhi, for research funding under the Young Scientist
Scheme (SB/YS/LS-12/2013) through SERB and INSPIRE Faculty
Fellowship Award (Grant No. IFA-LSPA-06). This work has been undertaken
as part of the CGIAR Research Program on Dryland Cereals,
and received funding from ICRISAT under the Blue Sky Research
Project on “Developing crops with high productivity under high temperatures”.
ICRISAT is a member of the CGIAR Consortium.
abstract: Pearl millet is a crop of the semi-arid tropics having high degree of genetic diversity and variable tolerance to drought stress. To investigate drought tolerance mechanism that possibly accounts for differences in drought tolerance, four recombinant inbred lines from a high resolution cross (HRC) were selected for variability in their transpiration rate (Tr) response to vapour pressure deficit (VPD) conditions. The differential Tr response of the genotypes to increased VPD conditions was used to classify the genotypes as sensitive or insensitive to high VPD. Aquaporin (AQP) genes PgPIP1;1, PgPIP1;2, PgPIP2;1, PgPIP2;3, PgPIP2;6, PgTIP1;1 and PgTIP2;2 were cloned. Phylogenetic analysis revealed that the cloned PgAQPs were evolutionarily closer to maize AQPs than to rice. PgAQP genes, including PgPIP1;1 and PgPIP2;6 in root tissue showed a significant expression pattern with higher expression in VPD-insensitive genotypes than VPD-sensitive genotypes under low VPD conditions (1.2 kPa) i.e when there is no high evaporative demand from the atmosphere. PgAQP genes (PgPIP2;1 in leaf and root tissues; PgPIP1;2 and PgTIP2;2 in leaf and PgPIP2;6 in root) followed a diurnal rhythm in leaves and roots that have either higher or lower expression levels at different time intervals. Under high VPD conditions (4.21 kPa), PgPIP2;3 showed higher transcript abundance in VPD-insensitive genotypes, and PgPIP2;1 in VPD-sensitive genotypes, while rest of the PgAQPs showed differential expression. Our current hypothesis is that these differences in the expression of AQP genes under different VPDs suggests a role of the AQPs in tuning the water transport pathways with variation between genotypes.
date: 2017-10
date_type: published
publication: Plant Science
volume: 265
publisher: Elsevier
pagerange: 167-176
id_number: 10.1016/j.plantsci.2017.10.005
refereed: TRUE
issn: 01689452
official_url: http://dx.doi.org/10.1016/j.plantsci.2017.10.005
related_url_url: https://scholar.google.co.in/scholar?hl=en&as_sdt=0%2C5&q=Molecular+cloning+and+expression+analysis+of+Aquaporin+genes+in+pearl+millet+%5BPennisetum+glaucum+%28L%29+R.+Br.%5D+genotypes+contrasting+in+their+transpiration+response+to+high+vapour+pressure+de
related_url_type: pub
funders: Department of Science and Technology, Govt. of India
projects: Blue Sky Research Project
citation:   Reddy, P S and Tharanya, M and Sivasakthi, K and Srikanth, M and Hash, C T and Kholova, J and Sharma, K K and Vadez, V  (2017) Molecular cloning and expression analysis of Aquaporin genes in pearl millet [ Pennisetum glaucum (L) R. Br.] genotypes contrasting in their transpiration response to high vapour pressure deficits.  Plant Science, 265.  pp. 167-176.  ISSN 01689452     
document_url: http://oar.icrisat.org/10234/1/Molecular%20cloning%20and%20expression%20analysis.pdf