eprintid: 10212
rev_number: 18
eprint_status: archive
userid: 1305
dir: disk0/00/01/02/12
datestamp: 2017-10-16 09:30:41
lastmod: 2018-07-03 09:10:23
status_changed: 2017-10-16 09:30:41
type: conference_item
metadata_visibility: show
creators_name: Santisree, P
creators_name: Bhatnagar-Mathur, P
creators_name: Sharma, K K
icrisatcreators_name: Santisree, P
icrisatcreators_name: Bhatnagar-Mathur, P
icrisatcreators_name: Sharma, K K
affiliation: ICRISAT (Patancheru)
country: India
title: Heat induced differential proteomic changes reveal molecular mechanisms responsible for heat tolerance in chickpea
ispublished: pub
subjects: ABio
subjects: DT1
subjects: MB1
subjects: s1.1
subjects: s1000
divisions: CRPS3
full_text_status: public
pres_type: paper
keywords: Molecular mechanisms, heat tolerance, chickpea, stress resilience, drought tolerance
abstract: Understanding the molecular differences in plant genotypes
contrasting for heat sensitivity can provide useful insights into
the mechanisms that confer heat tolerance in plants. We focused
on comparative physiological and proteomic analyses
of heat sensitive (ICC16374) and tolerant (JG14) genotypes
of chickpea (Cicer arietinum L.) when subjected to heat stress
at anthesis. Heat stress reduced seed germination, leaf water
content, chlorophyll content and membrane integrity with
a greater impact on sensitive genotype than on the tolerant
ones that had higher total antioxidant capacity and osmolyte
accumulation, and consequently less oxidative damage. Comparative
gel-free proteome profiles indicated differences in the
expression levels and regulation of common proteins that are
associated with heat tolerance in contrasting genotypes under
heat stress. Several crucial heat induced and heat responsive
proteins were identified and categorized based on ontology
and pathway analysis. The proteins which are essentially related
to the electron transport chain in photosynthesis, aminoacid
biosynthesis, ribosome synthesis and secondary metabolite
synthesis may play key roles in inducing heat tolerance.
In addition, our study also provides evidence that the foliar
application of nitric oxide (NO) donor can enhance heat and
drought stress tolerance by modulating a number of proteins
in chickpea. Understanding the active metabolic adjustments
in tolerant genotype under stress and inducing the stress tolerance
in sensitive genotype by exogenous NO application offers
a comprehensive and systematic approach to tackle heat and
drought stress in chickpea. This study potentially contributes
to improved stress resilience by offering valuable insights on
the mechanisms of heat and drought tolerance in chickpea.
date: 2017-02
date_type: published
pagerange: 219
event_title: InterDrought-V
event_location: Hyderabad, India
event_dates: February 21-25, 2017
event_type: conference
refereed: TRUE
official_url: http://idv.ceg.icrisat.org/wp-content/uploads/2017/02/Abstract_Book_Final.pdf
related_url_url: http://idv.ceg.icrisat.org/wp-content/uploads/2017/02/Abstract_Book_Final.pdf
related_url_type: org
citation:   Santisree, P and Bhatnagar-Mathur, P and Sharma, K K  (2017) Heat induced differential proteomic changes reveal molecular mechanisms responsible for heat tolerance in chickpea.  In: InterDrought-V, February 21-25, 2017, Hyderabad, India.     
document_url: http://oar.icrisat.org/10212/1/Abstract_Book_411.pdf