eprintid: 10172
rev_number: 15
eprint_status: archive
userid: 1305
dir: disk0/00/01/01/72
datestamp: 2017-09-05 04:57:03
lastmod: 2017-09-07 08:44:23
status_changed: 2017-09-05 04:57:03
type: article
metadata_visibility: show
creators_name: Nayak, S N
creators_name: Agarwal, G
creators_name: Pandey, M K
creators_name: Sudini, H
creators_name: Jayale, A S
creators_name: Purohit, S
creators_name: Desai, A
creators_name: Wan, L
creators_name: Guo, B
creators_name: Liao, B
creators_name: Varshney, R K
icrisatcreators_name: Nayak, S N
icrisatcreators_name: Agarwal, G
icrisatcreators_name: Pandey, M K
icrisatcreators_name: Sudini, H
icrisatcreators_name: Jayale, A S
icrisatcreators_name: Purohit, S
icrisatcreators_name: Desai, A
icrisatcreators_name: Varshney, R K
affiliation: ICRISAT (Patancheru)
affiliation: Crop Protection and Management Research Unit, USDA-Agricultural Research Service (Tifton)
affiliation: University of Georgia, Department of Plant Pathology (Tifton)
affiliation: Oil Crops Research Institute (OCRI), Chinese Academy of Agricultural Sciences (CAAS)(Wuhan)
affiliation: The University of Western Australia (Crawley)
affiliation: Department of Biotechnology, Present Address: University of Agricultural Sciences (Dharwad)
country: India
country: USA
country: China
country: Australia
title: Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization
ispublished: pub
subjects: S8
subjects: s1.3
subjects: s35
subjects: s40051
divisions: CRPS3
crps: crp1.5
full_text_status: public
keywords: Aflatoxin contamination, Host-pathogen, Groundnut, Aflatoxin estimation, Aspergillus infection, Aspergillus flavus infection
note: We acknowledge Dr. Manish Vishwakarma for designing allele specific primers and Ms. Vanika Garg for
preparing circos diagram for the manuscript. This research was partially funded by the USAID-US University
Collaboration Grant, Peanut & Mycotoxin Innovation Lab (PMIL), National Natural Science Foundation of
China (No. 31461143022) funded China-CGIAR Collaborative Research Project and MARS Chocolate North
America, USA. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes.
ICRISAT is a member of CGIAR Consortium.
abstract: Aflatoxin contamination, caused by fungal pathogen Aspergillus flavus, is a major quality and health problem delimiting the trade and consumption of groundnut (Arachis hypogaea L.) worldwide. RNA-seq approach was deployed to understand the host-pathogen interaction by identifying differentially expressed genes (DEGs) for resistance to in-vitro seed colonization (IVSC) at four critical stages after inoculation in J 11 (resistant) and JL 24 (susceptible) genotypes of groundnut. About 1,344.04 million sequencing reads have been generated from sixteen libraries representing four stages in control and infected conditions. About 64% and 67% of quality filtered reads (1,148.09 million) were mapped onto A (A. duranensis) and B (A. ipaёnsis) subgenomes of groundnut respectively. About 101 million unaligned reads each from J 11 and JL 24 were used to map onto A. flavus genome. As a result, 4,445 DEGs including defense-related genes like senescence-associated proteins, resveratrol synthase, 9s-lipoxygenase, pathogenesis-related proteins were identified. In A. flavus, about 578 DEGs coding for growth and development of fungus, aflatoxin biosynthesis, binding, transport, and signaling were identified in compatible interaction. Besides identifying candidate genes for IVSC resistance in groundnut, the study identified the genes involved in host-pathogen cross-talks and markers that can be used in breeding resistant varieties.
date: 2017-08-29
date_type: published
publication: Scientific Reports
volume: 7
number: 9659
publisher: Nature Publishing Group
pagerange: 1-14
id_number: 10.1038/s41598-017-09260-8
refereed: TRUE
issn: 2045-2322
official_url: http://dx.doi.org/10.1038/s41598-017-09260-8
related_url_url: https://scholar.google.co.in/scholar?q=Aspergillus+flavus+infection+triggered+immune+responses+and+host-pathogen+cross-talks+in+groundnut+during+in-vitro+seed+colonization&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
citation:   Nayak, S N and Agarwal, G and Pandey, M K and Sudini, H and Jayale, A S and Purohit, S and Desai, A and Wan, L and Guo, B and Liao, B and Varshney, R K  (2017) Aspergillus flavus infection triggered immune responses and host-pathogen cross-talks in groundnut during in-vitro seed colonization.  Scientific Reports, 7 (9659).  pp. 1-14.  ISSN 2045-2322     
document_url: http://oar.icrisat.org/10172/1/41598_2017_Article_9260.pdf