eprintid: 10171
rev_number: 13
eprint_status: archive
userid: 1305
dir: disk0/00/01/01/71
datestamp: 2017-09-05 04:30:08
lastmod: 2017-09-05 04:30:08
status_changed: 2017-09-05 04:30:08
type: article
metadata_visibility: show
creators_name: Xia, Y
creators_name: Li, R
creators_name: Bai, G
creators_name: Siddique, K H M
creators_name: Varshney, R K
creators_name: Baum, M
creators_name: Yan, G
creators_name: Guo, P
icrisatcreators_name: Varshney, R K
affiliation: International Crop Research Center for Stress Resistance/College of Life Sciences, Guangzhou University (Guangzhou)
affiliation: USDA-ARS Hard Winter Wheat Genetics Research Unit (Manhattan)
affiliation: The UWA Institute of Agriculture, The University of Western Australia (Perth)
affiliation: ICRISAT (Patancheru)
affiliation: International Center for Agricultural Research in the Dry Areas (ICARDA) (Beirut)
affiliation: School of Agriculture and Environment, The University of Western Australia (Perth)
country: China
country: USA
country: Australia
country: India
country: Lebanon
title: Genetic variations of HvP5CS1 and their association with drought tolerance related traits in barley (Hordeum vulgare L.)
ispublished: pub
subjects: S1
subjects: s1000
subjects: s2.13
subjects: s2.8
divisions: CRPS3
full_text_status: public
keywords: Genetic variations, Drought tolerance, Barley, Barley collection
note: This work was funded by the Provincial Key International Cooperative Research Platform and the Major Scientific
Research Project of Guangdong Higher Education (2015KGJHZ015), and the Science and Technology Plan of
Guangdong of China (2016B020201001).
abstract: Delta-1-pyrroline-5-carboxylate synthase gene1 (P5CS1) is the key gene involved in the biosynthesis of proline and is significantly induced by drought stress. The exploration of genetic variation in HvP5CS1 may facilitate a better understanding of the mechanism of drought adaptation in barley. In the current study, 41 polymorphisms including 16 single nucleotide polymorphisms (SNPs) and 25 insertions/deletions (indels) were detected in HvP5CS1 among 287 barley (Hordeum vulgare L.) accessions collected worldwide, with 13 distinct haplotypes identified in the barley collection. Five polymorphisms in HvP5CS1 were significantly (P < 0.001) associated with drought tolerance related traits in barley. The phenotypic variation of a given trait explained by each associated polymorphism ranged from 4.43% to 9.81%. Two sequence variations that were significantly (P < 0.0001) associated with grain yield had marginally significant positive Tajima’s D values in the sliding window, so they might have been selected for environmental adaptation. Meanwhile, two haplotypes HvP5CS1_H1 and HvP5CS1_H4, which contained desired alleles of the two variations mentioned above, were significantly (P < 0.001) associated with drought tolerance related traits, and explained 5.00~11.89% of the phenotypic variations. These variations associated with drought tolerance related traits can be used as potential markers for improving drought tolerance in barley.
date: 2017-08-11
date_type: published
publication: Scientific Reports
volume: 7
number: 7870
publisher: Springer Nature
pagerange: 1-10
id_number: 10.1038/s41598-017-08393-0
refereed: TRUE
issn: 2045-2322
official_url: http://dx.doi.org/10.1038/s41598-017-08393-0
related_url_url: https://scholar.google.co.in/scholar?hl=en&q=Genetic+variations+of+HvP5CS1+and+their+association+with+drought+tolerance+related+traits+in+barley+%28Hordeum+vulgare+L.%29&btnG=
related_url_type: pub
citation:   Xia, Y and Li, R and Bai, G and Siddique, K H M and Varshney, R K and Baum, M and Yan, G and Guo, P  (2017) Genetic variations of HvP5CS1 and their association with drought tolerance related traits in barley (Hordeum vulgare L.).  Scientific Reports, 7 (7870).  pp. 1-10.  ISSN 2045-2322     
document_url: http://oar.icrisat.org/10171/1/s41598-017-08393-0.pdf