eprintid: 10129
rev_number: 11
eprint_status: archive
userid: 1305
dir: disk0/00/01/01/29
datestamp: 2017-08-07 10:06:02
lastmod: 2017-08-10 06:41:32
status_changed: 2017-08-07 10:06:02
type: article
metadata_visibility: show
contact_email: Library-ICRISAT@CGIAR.ORG
creators_name: Santisree, P
creators_name: Bhatnagar-Mathur, P
creators_name: Sharma, K K
icrisatcreators_name: Santisree, P
icrisatcreators_name: Bhatnagar-Mathur, P
icrisatcreators_name: Sharma, K K
affiliation: ICRISAT (Patancheru)
country: India
title: Molecular insights into the functional role of nitric oxide (NO) as a signal for plant responses in chickpea
ispublished: pub
subjects: MB1
subjects: s1.1
subjects: s2.13
divisions: CRPS3
crps: crp1.5
full_text_status: restricted
keywords: Abiotic stress, Gel-free proteomics, Mass spectroscopy, Sodium nitroprusside, Nitric oxide, Chickpea
note: PS thanks ICRISAT for hosting and providing research facilities. This
work was undertaken as part of the CGIAR Research Program on Grain
Legumes. This work was supported by financial support to PS through
the INSPIRE Faculty Award (IFA12-LSPA-08) from the Department of
Science and Technology, Government of India and partial funding from
the CGIAR Research Program on Grain Legumes to KKS.
abstract: The molecular mechanisms and targets of nitric oxide (NO) are not fully known in plants. Our study reports the first large-scale quantitative proteomic analysis of NO donor responsive proteins in chickpea. Dose response studies carried out using NO donors, sodium nitroprusside (SNP), diethylamine NONOate (DETA) and S-nitrosoglutathione (GSNO) in chickpea genotype ICCV1882, revealed a dose dependent positive impact on seed germination and seedling growth. SNP at 0.1 mM concentration proved to be most appropriate following confirmation using four different chickpea genotypes. while SNP treatment enhanced the percentage of germination, chlorophyll and nitrogen contents in chickpea, addition of NO scavenger, cPTIO reverted its impact under abiotic stresses. Proteome profiling revealed 172 downregulated and 76 upregulated proteins, of which majority were involved in metabolic processes (118) by virtue of their catalytic (145) and binding (106) activity. A few crucial proteins such as S-adenosylmethionine synthase, dehydroascorbate reductase, pyruvate kinase fragment, 1-aminocyclopropane-1-carboxylic acid oxidase, 1-pyrroline-5-carboxylate synthetase were less abundant whereas Bowman-Birk type protease inhibitor, non-specific lipid transfer protein, chalcone synthase, ribulose-1-5-bisphosphate carboxylase oxygenase large subunit, PSII D2 protein were highly abundant in SNP treated samples. This study highlights the protein networks for a better understanding of possible NO induced regulatory mechanisms in plants.
date: 2017-04-13
date_type: published
publication: Functional Plant Biology
publisher: CSIRO
pagerange: 1-17
id_number: 10.1071/FP16324
refereed: TRUE
issn: 1445-4408
official_url: http://dx.doi.org/10.1071/FP16324
related_url_url: https://scholar.google.co.in/scholar?q=Molecular+insights+into+the+functional+role+of+nitric+oxide+%28NO%29+as+a+signal+for+plant+responses+in+chickpea&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
funders: Department of Science and Technology, Government of India & CGIAR Research Program on Grain Legumes
citation:   Santisree, P and Bhatnagar-Mathur, P and Sharma, K K  (2017) Molecular insights into the functional role of nitric oxide (NO) as a signal for plant responses in chickpea.  Functional Plant Biology.  pp. 1-17.  ISSN 1445-4408     
document_url: http://oar.icrisat.org/10129/1/FPB.pdf