"10107","8","archive","1305",,,"disk0/00/01/01/07","2017-07-26 10:38:44","2017-07-26 10:38:44","2017-07-26 10:38:44","article",,,"show","Library-ICRISAT@CGIAR.ORG",,,"","","","","","","","","","",,,,"Pratap","A","","","","","","","Thudi","M","","","","",,,,,"","",,,,,"","","Crop Improvement Division, ICAR-Indian Institute of Pulses Research (Kanpur)","India","Marker-assisted introgression of resistance to fusarium wilt race 2 in Pusa 256, an elite cultivar of desi chickpea","pub","s1.1","CRPS3","","restricted",,,"Backcrossing, Chickpea, BGS, FGS, MABC, Molecular breeding, SSR markers , Fusarium wilt, Desi chickpea",,"The authors would like to thank Manish K. Pandey,
Siva K. Chamarthi (ICRISAT), Subhojit Datta (ICAR-IIPR) and
partners in ACIP programme from JNKVV, Jabalpur, ARS, Kalaburgi
and MPKV, Rahuri for useful discussions during the course of this
study. The authors are also highly thankful to Col Douglas, Senior
Research Scientist, Agri-Science, Queensland, Australia for reading
the manuscript for language corrections. The present study was supported under the Accelerated
Crop Improvement Programme (ACIP) of Department of Biotechnology
(DBT), Government of India vide Grant No. BT/PR11693/
AGR/02/641/2008, 22 October 2009. All laboratory and field facilities
were provided by ICAR-IIPR Kanpur.","Fusarium wilt caused by F. oxysporum f. sp. ciceris
causes extensive damage to chickpea (Cicer arietinum
L.) in many parts of the world. In the central part of India,
pathogen race 2 (Foc 2) causes severe yield losses. We initiated
molecular marker-assisted backcrossing (MABC)
using desi cultivar, Vijay, as a donor to introgress resistance
to this race (Foc2) in Pusa 256, another elite desi cultivar
of chickpea. To confirm introgression of resistance for this
race, foreground selection was undertaken using two SSR
markers (TA 37 and TA110), with background selection to
observe the recovery of recurrent parent genome using 45
SSRs accommodated in 8 multiplexes. F1
plants were confirmed
with molecular markers and backcrossed with Pusa
256, followed by cycles of foreground and background
selection at each stage to generate 161 plants in BC3F2
during the period 2009–2013. Similarly, 46 BC3F1
plants
were also generated in another set during the same period.
On the basis of foreground selection, 46 plants were found
homozygotes in BC3F2. Among them, 17 plants recorded
>91% background recovery with the highest recovery percentage
of 96%. In BC3F1
also, 14 hybrid plants recorded a
background recovery of >85% with the highest background
recovery percentage of >94%. The identified plants were
selfed to obtain 1341 BC3F3
and 2198 BC3F2
seeds which
were screened phenotypically for resistance to fusarium
wilt (race 2) besides doing marker analysis. Finally, 17
BC3F4
and 11 BC3F3
lines were obtained which led to identification
of 5 highly resistant lines of Pusa 256 with Foc 2
gene introgressed in them. Development of these lines will
help in horizontal as well as vertical expansion of chickpea
in central part of India.","2017-07","published",,"Molecular Genetics and Genomics",,,"Springer",,"1-9",,,,,,"10.1007/s00438-017-1343-z",,,,,"TRUE",,"1617-4615",,,,,,"","http://dx.doi.org/10.1007/s00438-017-1343-z","https://scholar.google.co.in/scholar?hl=en&q=Marker-assisted+introgression+of+resistance+to+fusarium+wilt+race+2+in+Pusa+256%2C+an+elite+cultivar+of+desi+chickpea&btnG=","pub",,"","",,,,,,"",,,,,,,"",,,,,"",,,,,"","",,,,,"","",,,,,
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