eprintid: 10099
rev_number: 14
eprint_status: archive
userid: 1305
dir: disk0/00/01/00/99
datestamp: 2017-07-21 05:27:08
lastmod: 2017-08-03 03:26:54
status_changed: 2017-07-21 05:27:08
type: article
metadata_visibility: show
creators_name: Srikanth, S
creators_name: Swathi, M
creators_name: Kollipara, P
creators_name: Rao, M V
creators_name: Mallikarjuna, N
icrisatcreators_name: Srikanth, S
icrisatcreators_name: Mallikarjuna, N
affiliation: ICRISAT (Patancheru)
affiliation: Bharathidasan University (Thiruchirappalli)
affiliation: Department of Plant Sciences, School of Life Sciences, University of Hyderabad (Hyderabad)
affiliation: Department of Biotechnology and Bioinformatics, School of Life Sciences, University of Hyderabad (Hyderabad)
country: India
title: Protease inhibitors of Cajanus conferring resistance to pod borer of pigeonpea (Cajanus cajan L. Millsp.).
ispublished: pub
subjects: S8
subjects: h1
subjects: s1.2
subjects: s2.13
divisions: CRPS3
full_text_status: public
keywords: Cajanus, Cajanus cajan, Helicoverpa, Helicoverpa armigera, insects, plants, Chymotrypsin, trypsin, Human pancreatic trypsin, midgut trypsin-like proteases, pigeonpea, pod borer, protease inhibitors
abstract: Pigeonpea is susceptible to pod borer damage with resistance lacking in its primary gene pool. Many Cajanus species harbor high levels of resistance. Host plant resistance can play an important role in minimizing the extent of losses due to insects and pests as well as the use of insecticides/pesticides and thus protect the environment. A major initiative was undertaken to tap the defence genes from wild relatives of secondary and tertiary gene pool through wide hybridization and thereby introgress resistance to pod borer. A range of interspecific derivatives derived from C. lanceolatus, C. cajanifolius, C. volubilis and C. platycarpus along with their parents were screened for the pod borer resistance under unprotected field conditions at ICRISAT, Patancheru, India. Biochemical basis of resistance was also identified by studying the levels of defence proteins active against bovine pancreatic trypsin, chymotrypsin and trypsin-like enzymes of H. armigera mid-gut proteases. Protease inhibitor profiles of parents and interspecific derivatives differed in terms of activity units, number and intensities of activity bands visualized on gelatin-PAGE. As the protease inhibitors are anti-nutritional factors, parents and interspecific derivatives, which resulted in high levels of Helicoverpa gut protease inhibitor (HGPI) units were screened for Human pancreatic trypsin inhibitor (HPTI) activity levels. Samples with high ratio of HGPI/HPTI represent less or no effect on human pancreatic trypsin and high effect on insect gut proteases.
date: 2017-03
date_type: published
publication: Electronic Journal of Plant Breeding
volume: 8
number: 1
publisher: Indian Society of Plant Breeders
pagerange: 26-37
refereed: TRUE
issn: 0975-928X
official_url: http://ejplantbreeding.com/index.php?journal=EJPB&page=article&op=view&path%5B%5D=1211&path%5B%5D=999
related_url_url: https://scholar.google.co.in/scholar?q=Protease+inhibitors+of+Cajanus+conferring+resistance+to+pod+borer+of+pigeonpea+%28Cajanus+cajan+L.+Millsp.%29&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
citation:   Srikanth, S and Swathi, M and Kollipara, P and Rao, M V and Mallikarjuna, N  (2017) Protease inhibitors of Cajanus conferring resistance to pod borer of pigeonpea (Cajanus cajan L. Millsp.).  Electronic Journal of Plant Breeding, 8 (1).  pp. 26-37.  ISSN 0975-928X     
document_url: http://oar.icrisat.org/10099/1/Electronic%20Journal%20of%20Plant%20Breeding.pdf