eprintid: 10065
rev_number: 12
eprint_status: archive
userid: 1305
dir: disk0/00/01/00/65
datestamp: 2017-06-29 09:53:15
lastmod: 2017-06-29 09:54:10
status_changed: 2017-06-29 09:53:15
type: article
metadata_visibility: show
creators_name: Siddaiah, C N
creators_name: Satyanarayana, N R
creators_name: Mudili, V
creators_name: Kumar Gupta, V
creators_name: Gurunathan, S
creators_name: Rangappa, S
creators_name: Huntrike, S S
creators_name: Srivastava, R K
icrisatcreators_name: Srivastava, R K
affiliation: Department of Studies in Biotechnology, University of Mysore, Manasagangotri (Mysore)
affiliation: Department of Studies in Microbiology, Karnataka State Open University, Mukthagangotri (Mysore)
affiliation: Microbiology Division, DRDO-BU-Centre for Life sciences, Bharathiar University Campus (Coimbatore)
affiliation: Discipline of Biochemistry, National University of Ireland Galway (Galway)
affiliation: Frontier Research Center for Post-Genome Science and Technology, Hokkaido University (Sapporo)
affiliation: ICRISAT (Patancheru)
country: India
country: Ireland
country: Japan
title: Elicitation of resistance and associated defense responses in Trichoderma hamatum induced protection against pearl millet downy mildew pathogen
ispublished: pub
subjects: S1.5.1
subjects: s35
divisions: CRPS3
crps: crp1.4
full_text_status: public
keywords: Pearl millet, Biochemical studies, Gene expression studies, T. hamatum, Downy mildew pathogen, Plant disease
note: The authors are grateful to ICAR (Indian Council of Agricultural Research), New Delhi, India for providing the financial support under AICRP scheme.
abstract: Endophytic Trichoderma hamatum UoM 13 isolated from pearl millet roots was evaluated for its efficiency to suppress downy mildew disease. Under laboratory conditions, T. hamatum seed treatment significantly enhanced pearl millet seed germination and seedling vigor. T. hamatum seed treatment resulted in systemic and durable immunity against pearl millet downy mildew disease under greenhouse and field conditions. T. hamatum treated seedlings responded to downy mildew infection with high lignification and callose deposition. Analysis of defense enzymes showed that T. hamatum treatment significantly enhanced the activities of glucanase, peroxidase, phenylalanine ammonia-lyase, and polyphenol oxidase in comparison to untreated control. RT-PCR analysis revealed differentially expressed transcripts of the defense enzymes and PR-proteins in treated, untreated, and checks, wherein PR-1, PR-5, and cell wall defense HRGPs were significantly over expressed in treated seedlings as against their lower expression in controls. T. hamatum treatment significantly stimulated endogenous salicylic acid (SA) levels and significantly upregulated important SA biosynthesis gene isochorismate synthase. The results indicated that T. hamatum UoM13 treatment induces resistance corresponding to significant over expression of endogenous SA, important defense enzymes, PR-proteins, and HRGPs, suggesting that SA biosynthetic pathway is involved in pearl millet for mounting systemic immunity against downy mildew pathogen.
date: 2017-03
date_type: published
publication: Scientific Reports
volume: 7
number: 43991
publisher: Nature Publishing Group
pagerange: 1-18
id_number: 10.1038/srep43991
refereed: TRUE
issn: 2045-2322
official_url: http://dx.doi.org/10.1038/srep43991
related_url_url: https://scholar.google.co.in/scholar?q=Elicitation+of+resistance+and+associated+defense+responses+in+Trichoderma+hamatum+induced+protection+against+pearl+millet+downy+mildew+pathogen&btnG=&hl=en&as_sdt=0%2C5
related_url_type: pub
funders: ICAR
citation:   Siddaiah, C N and Satyanarayana, N R and Mudili, V and Kumar Gupta, V and Gurunathan, S and Rangappa, S and Huntrike, S S and Srivastava, R K  (2017) Elicitation of resistance and associated defense responses in Trichoderma hamatum induced protection against pearl millet downy mildew pathogen.  Scientific Reports, 7 (43991).  pp. 1-18.  ISSN 2045-2322     
document_url: http://oar.icrisat.org/10065/1/srep43991.pdf